NDLI: Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus.

Please wait, while we are loading the content...

Adenovirus type 5 with modified hexons induces robust transgene-specific immune responses in mice with pre-existing immunity against adenovirus type 5.

Intranuclear fluorescence resonance energy transfer analysis of plasmid DNA decondensation from nonviral gene carriers.

Osteoinduction by microbubble-enhanced transcutaneous sonoporation of human bone morphogenetic protein-2.

Enhanced transgene expression in the mouse skeletal muscle infected by the adeno-associated viral vector with the human elongation factor 1alpha promoter and a human chromatin insulator.

In vivo secretion of the mouse immunoglobulin G Fc fragment from rat submandibular glands.

Inflammatory cell-specific transgene expression system responding to Ikappa-B kinase beta activation.

Neuron-specific RNA interference using lentiviral vectors.

Redirecting lentiviral vectors by insertion of integrin-tageting peptides into envelope proteins.

Hepatic insulin gene therapy diminishes liver glycogen despite insulin responsive transcriptional effects in diabetic CD-1 mice.

Year: 2009 Volume: 11 Issue: 6

Year: 2009 Volume: 11 Issue: 5

Year: 2009 Volume: 11 Issue: 4

Year: 2009 Volume: 11 Issue: 3

Year: 2009 Volume: 11 Issue: 2

Year: 2009 Volume: 11 Issue: 1

Year: 2008 Volume: 10

Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kay, Jesse D. Gouze, Elvire Oligino, Thomas J. Gouze, Jean-Noel Watson, Rachael S. Levings, Padraic P. Bush, Marsha L. Dacanay, Anthony Nickerson, David M. Robbins, Paul D. Evans, Christopher H. Ghivizzani, Steven C.
Description	Country affiliation: United States Author Affiliation: Kay JD ( Department of Orthopaedics and Rehabilitation, University of Florida College of Medicine, Gainesville, 32610-0137, USA.)
Abstract	BACKGROUND: The adeno-associated virus (AAV) has many safety features that favor its use in the treatment of arthritic conditions; however, the conventional, single-stranded vector is inefficient for gene delivery to fibroblastic cells that primarily populate articular tissues. This has been attributed to the inability of these cells to convert the vector to a double-stranded form. To overcome this, we evaluated double-stranded self-complementary (sc) AAV as a vehicle for intra-articular gene delivery. METHODS: Conventional and scAAV vectors were used to infect lapine articular fibroblasts in culture to determine transduction efficiency, transgene expression levels, and nuclear trafficking. scAAV containing the cDNA for interleukin (IL)-1 receptor antagonist (Ra) was delivered to the joints of naïve rabbits and those with IL-1beta-induced arthritis. From lavage of the joint space, levels of transgenic expression and persistence were measured by enzyme-linked immunosorbent assay. Infiltrating leukocytes were quantified using a hemocytometer. RESULTS: Transgene expression from scAAV had an earlier onset and was approximately 25-fold greater than conventional AAV despite the presence of similar numbers of viral genomes in the nuclei of infected cells. Fibroblasts transduced with scAAV produced amounts of IL1-Ra comparable to those transduced with adenoviral and lentiviral vectors. IL1-Ra was present in lavage fluid of most animals for 2 weeks in sufficient quantities to inhibit inflammation of the IL-1beta-driven model. Once lost, neither subsequent inflammatory events, nor re-administration of the virus could re-establish transgene expression. CONCLUSIONS: scAAV-mediated intra-articular gene transfer is robust and similarly efficient in both normal and inflamed joints; the resulting transgenic expression is sufficient to achieve biological relevance in joints of human proportion.
File Format	HTM / HTML
ISSN	1099498X
e-ISSN	15212254
DOI	10.1002/jgm.1334
Journal	The Journal of Gene Medicine
Issue Number	7
Volume Number	11
Language	English
Publisher	Wiley
Publisher Date	2009-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Dependovirus Genetics Gene Transfer Techniques Genetic Therapy Injections, Intra-articular Interleukin 1 Receptor Antagonist Protein Animals Arthritis Therapy Cartilage, Articular Cytology Cells, Cultured Metabolism Fibroblasts Physiology Genetic Vectors Rabbits Transgenes Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Genetics Drug Discovery Molecular Biology Molecular Medicine Genetics (clinical)

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus

Intraarticular expression of biologically active interleukin 1-receptor-antagonist protein by ex vivo gene transfer.

Adeno-associated virus-mediated IL-10 gene transfer suppresses lacrimal gland immunopathology in a rabbit model of autoimmune dacryoadenitis.

Adeno-associated virus-mediated microRNA delivery and therapeutics.

Adeno-associated virus 8-mediated gene therapy for choroideremia: preclinical studies in in vitro and in vivo models.

Ex vivo gene delivery to synovium using foamy viral vectors.

Local delivery of beta interferon using an adeno-associated virus type 5 effectively inhibits adjuvant arthritis in rats.

Effect of recombinant adeno-associated BMP-4/7 fusion gene on the biology of BMSCs.

Gene Transfer to Mouse Kidney In Vivo

Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus.

Similar Documents

Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus

Intraarticular expression of biologically active interleukin 1-receptor-antagonist protein by ex vivo gene transfer.

Adeno-associated virus-mediated IL-10 gene transfer suppresses lacrimal gland immunopathology in a rabbit model of autoimmune dacryoadenitis.

Adeno-associated virus-mediated microRNA delivery and therapeutics.

Adeno-associated virus 8-mediated gene therapy for choroideremia: preclinical studies in in vitro and in vivo models.

Ex vivo gene delivery to synovium using foamy viral vectors.

Local delivery of beta interferon using an adeno-associated virus type 5 effectively inhibits adjuvant arthritis in rats.

Effect of recombinant adeno-associated BMP-4/7 fusion gene on the biology of BMSCs.

Gene Transfer to Mouse Kidney In Vivo

Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus.