NDLI: A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide.

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A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Morizono, Kouki Xie, Yiming Helguera, Gustavo Daniels, Tracy R. Lane, Timothy F. Penichet, Manuel L. Chen, Irvin S. Y.
Description	Country affiliation: United States Author Affiliation: Morizono K ( Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.)
Abstract	BACKGROUND: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. METHODS: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. RESULTS: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. CONCLUSIONS: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin.
File Format	HTM / HTML
ISSN	1099498X
e-ISSN	15212254
DOI	10.1002/jgm.1345
Journal	The Journal of Gene Medicine
Issue Number	8
Volume Number	11
Language	English
Publisher	Wiley
Publisher Date	2009-08-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Biotin Metabolism Gene Transfer Techniques Genetic Vectors Genetics Lentivirus Peptides Avidin Biotinylation Blotting, Western Cell Line Electrophoresis, Polyacrylamide Gel Magnetics Mutagenesis, Insertional Receptors, Transferrin Recombinant Fusion Proteins Sindbis Virus Transduction, Genetic Viral Envelope Proteins Virion Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Genetics Drug Discovery Molecular Biology Molecular Medicine Genetics (clinical)

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Redirecting lentiviral vectors by insertion of integrin-tageting peptides into envelope proteins.

A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide

Targeting lentiviral vectors to specific cell types in vivo.

Envelope protein-specific B cell receptors direct lentiviral vector tropism invivo

Rabies virus envelope glycoprotein targets lentiviral vectors to the axonal retrograde pathway in motor neurons.

Efficient gene therapy based targeting system for the treatment of inoperable tumors.

An avidin-like domain that does not bind biotin is adopted for oligomerization by the extracellular mosaic protein fibropellin.

Development of adenovirus immobilization strategies for in situ gene therapy.

Oligomeric structure of virion-associated and soluble forms of the simian immunodeficiency virus envelope protein in the prefusion activated conformation.

A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide.

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