NDLI: A survey of parameters involved in the establishment of new lines of human embryonic stem cells.

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Year: 2011 Volume: 7 Issue: 3

Year: 2011 Volume: 7 Issue: 2

Year: 2011 Volume: 7 Issue: 1

A survey of parameters involved in the establishment of new lines of human embryonic stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fraga, Ana Maria Souza de Araújo, Érica Sara Stabellini, Raquel Vergani, Naja Pereira, Lygia V.
Description	Country affiliation: Brazil Author Affiliation: Fraga AM ( Laboratório Nacional de Células-Tronco Embrionárias and Instituto Nacional de Ciência e Tecnologia em Células-Tronco e Terapia Celular, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP 05508-090, Brazil.)
Abstract	Since the derivation of the first human embryonic stem cell (hESC) lines by Thomson and coworkers in 1998, more than 1,200 different hESC lines have been established worldwide. Nevertheless, there is still a recognized interest in the establishment of new lines of hESC, particularly from HLA types and ethnic groups currently underrepresented among the available lines. The methodology of hESC derivation has evolved significantly since 1998, when human LIF (hLIF) was used for maintenance of pluripotency. However, there are a number of different strategies for the several steps involved in establishing a new line of hESC. Here we make a survey of the most relevant parameters used between 1998 and 2010 for the derivation of the 375 hESC lines deposited in two international stem cell registries, and able to form teratomas in immunocompromised mice. Although we identify some trends in the methodology for establishing hESC lines, our data reveal a much greater heterogeneity of strategies than what is used for derivation of murine ESC lines, indicating that optimum conditions have not been consolidated yet, and thus, hESC establishment is still an evolving field of research.
File Format	HTM / HTML
ISSN	15508943
Issue Number	4
Volume Number	7
e-ISSN	15586804
Journal	Stem Cell Reviews and Reports
Language	English
Publisher	Springer
Publisher Date	2011-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Cell Biology Cell Culture Techniques Methods Standards Cell Line Embryonic Stem Cells Cytology Animals Biological Specimen Banks Culture Media Chemistry Embryo Culture Techniques Embryo, Mammalian Pathology Humans Mice Pluripotent Stem Cells Quality Control Statistics As Topic Teratoma Journal Article Research Support, Non-u.s. Gov't Review
Content Type	Text
Resource Type	Article
Subject	Medicine

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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A survey of parameters involved in the establishment of new lines of human embryonic stem cells.

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