NDLI: Simple and rapid enzymatic method for the synthesis of single-strand oligonucleotides containing trifluorothymidine.

Please wait, while we are loading the content...

Semisynthesis of 6-chloropurine-2'-deoxyriboside 5'-dimethoxytrityl 3'-(2-cyanoethyl-N,N-diisopropylamino)phosphoramidite and its use in the synthesis of fluorescently labeled oligonucleotides.

Quadruplex-duplex motifs as new topoisomerase I inhibitors.

Study on the interaction between the inclusion complex of hematoxylin with ß-cyclodextrin and DNA.

Gas-phase cleavage and dephosphorylation of universal linker-bound oligodeoxynucleotides.

Labeled nucleoside triphosphates with reversibly terminating aminoalkoxyl groups.

Simple and rapid enzymatic method for the synthesis of single-strand oligonucleotides containing trifluorothymidine.

Synthesis of novel 4'-cyclopropyl-5'-norcarbocyclic adenosine phosphonic acid analogues.

Year: 2010 Volume: 29 Issue: 10

Year: 2010 Volume: 29 Issue: 9

Year: 2010 Volume: 29 Issue: 8

Year: 2010 Volume: 29 Issue: 7

Year: 2010 Volume: 29 Issue: 3

Year: 2010 Volume: 29 Issue: 2

Year: 2010 Volume: 29 Issue: 1

Year: 2010 Volume: 29 Issue: 4-6

Year: 2009 Volume: 28

Year: 2008 Volume: 27

Year: 2007 Volume: 26

Year: 2006 Volume: 25

Year: 2005 Volume: 24

Year: 2001 Volume: 20

Simple and rapid enzymatic method for the synthesis of single-strand oligonucleotides containing trifluorothymidine.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Suzuki, Norihiko Fukushima, Masakazu
Description	Country affiliation: Japan Author Affiliation: Suzuki N ( Tokushima Research Center, Taiho Pharmaceutical Co. Ltd., 224-2 Hiraishi-ebisuno, Kawauchi, Tokushima, Japan. suzuki@taiho.co.jp)
Abstract	To investigate the mechanism of trifluorothymidine (TFT)-induced DNA damage, we developed an enzymatic method for the synthesis of single-strand oligonucleotides containing TFT-monophosphate residues. Sixteen-mer oligonucleotides and 14-mer 5'-phosphorylated oligonucleotides were annealed to the template of 25-mer, so as to empty one nucleotide site. TFT-triphosphate was incorporated into the site by DNA polymerase and then ligated to 5'-phosphorylated oligonucleotides by DNA ligase. The synthesized 31-mer oligonucleotides containing TFT residues were isolated from the 25-mer complementary template by denaturing polyacrylamide electrophoresis. Using these single-strand oligonucleotides containing TFT residues, the cleavage of TFT residues from DNA, using mismatch uracil-DNA glycosylase (MUG) of E.coli origin, was compared with that of 5-fluorouracil (5FU) and 5-bromodeoxyuridine (BrdU). The TFT/A pair was not cleaved by MUG, while the other pairs, namely, 5FU/A, 5FU/G, BrdU/A, BrdU/G, and TFT/G, were easily cleaved from each synthesized DNA. Thus, this method is useful for obtaining some site-specifically modified oligonucleotides.
File Format	HTM / HTML
ISSN	15257770
e-ISSN	15322335
DOI	10.1080/15257770.2010.535803
Journal	Nucleosides, Nucleotides and Nucleic Acids
Issue Number	11
Volume Number	29
Language	English
Publisher	Taylor & Francis
Publisher Date	2010-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Dna Damage Oligonucleotides Chemical Synthesis Trifluridine Chemistry Autoradiography Bromodeoxyuridine Dna Ligases Dna Primers Genetics Dna-directed Dna Polymerase Denaturing Gradient Gel Electrophoresis Fluorouracil Thymine Dna Glycosylase
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Simple and Rapid Enzymatic Method for the Synthesis of Single-Strand Oligonucleotides Containing Trifluorothymidine

RNA sequencing by primer extension.

Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis

Detection of competing DNA structures by thermal gradient gel electrophoresis: from self-association to triple helix formation by (G,A)-containing oligonucleotides

A simple method for identification of point mutations using denaturing gradient gel electrophoresis.

Methanobacterium thermoformicicum thymine DNA mismatch glycosylase: conversion of an N-glycosylase to an AP lyase

Rapid detection of the highly polymorphic beta globin framework by denaturing gradient gel electrophoresis.

Denaturing gradient gel electrophoresis for nonlethal detection of Aeromonas salmonicida in salmonid mucus and its potential for other bacterial fish pathogens.

Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.

Simple and rapid enzymatic method for the synthesis of single-strand oligonucleotides containing trifluorothymidine.

Similar Documents

Simple and Rapid Enzymatic Method for the Synthesis of Single-Strand Oligonucleotides Containing Trifluorothymidine

RNA sequencing by primer extension.

Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis

Detection of competing DNA structures by thermal gradient gel electrophoresis: from self-association to triple helix formation by (G,A)-containing oligonucleotides

A simple method for identification of point mutations using denaturing gradient gel electrophoresis.

Methanobacterium thermoformicicum thymine DNA mismatch glycosylase: conversion of an N-glycosylase to an AP lyase

Rapid detection of the highly polymorphic beta globin framework by denaturing gradient gel electrophoresis.

Denaturing gradient gel electrophoresis for nonlethal detection of Aeromonas salmonicida in salmonid mucus and its potential for other bacterial fish pathogens.

Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.

Simple and rapid enzymatic method for the synthesis of single-strand oligonucleotides containing trifluorothymidine.