NDLI: Allele-specific primer polymerase chain reaction for a single nucleotide polymorphism (C1205T) of swine toll-like receptor 5 and comparison of the allelic frequency among several pig breeds in Japan and the Czech Republic.

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Allele-specific primer polymerase chain reaction for a single nucleotide polymorphism (C1205T) of swine toll-like receptor 5 and comparison of the allelic frequency among several pig breeds in Japan and the Czech Republic.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Muneta, Yoshihiro Minagawa, Yu Kusumoto, Masahiro Shinkai, Hiroki Uenishi, Hirohide Splichal, Igor
Spatial Coverage	Japan Czech Republic
Description	Author Affiliation: Muneta Y ( Pathology and Pathophysiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5, Kannondai, Tsukuba, Ibaraki. ymuneta@affrc.go.jp)
Abstract	In the present study, an allele-specific primer-polymerase chain reaction (ASP-PCR) for genotyping a single nucleotide polymorphism (SNP) of swine Toll-like receptor 5 (TLR5) (C1205T; P402L) that is related to the impaired recognition of Salmonella enterica serovar Choleraesuis (SC) was developed. The allele frequencies in several pig breeds in Japan and the Czech Republic were also compared. The swine TLR5 C1205T mutation was successfully determined by ASP-PCR using genomic DNA samples in Japan that had previously been genotyped by a sequencing method. Using the PCR condition determined, genomic DNA samples from blood obtained from 110 pigs from seven different breeds in the Czech Republic were genotyped by the ASP-PCR. The genotyping results from the ASP-PCR completely matched the results from the sequencing method. The allele frequency of the swine TLR5 C1205T mutation was 27.5% in the Landrace breed of the Czech Republic compared with 50.0% in Japanese Landrace. In Japan, the C1205T mutation was found only in the Landrace breed, whereas in the Czech Republic it was found in both the Landrace and Piétrain breeds. These results indicate the usefulness of ASP-PCR for detecting a specific SNP for swine TLR5 affecting ligand recognition. They also suggest the possibility of genetically improving pigs to enhance their resistance against SC infection by eliminating or selecting this specific SNP of swine TLR5.
File Format	HTM / HTML
ISSN	03855600
Issue Number	6
Volume Number	56
e-ISSN	13480421
Journal	Microbiology and Immunology
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2012-06-01
Publisher Place	Australia
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Immunology Genetic Predisposition To Disease Genetic Testing Methods Polymerase Chain Reaction Polymorphism, Single Nucleotide Toll-like Receptor 5 Genetics Animals Czech Republic Gene Frequency Genotype Japan Salmonella Infections, Animal Salmonella Enterica Immunology Swine Swine Diseases Comparative Study Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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