NDLI: Effect of HIF1 on Foxp3 expression in CD4+ CD25- T lymphocytes.

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Effect of HIF1 on Foxp3 expression in CD4+ CD25- T lymphocytes.

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Effect of HIF1 on Foxp3 expression in CD4+ CD25- T lymphocytes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wu, Jianguo Cui, Hongyan Zhu, Zhifeng Wang, Li Li, Hong Wang, Desheng
Description	Author Affiliation: Wu J ( The Second Hospital of Tianjin Medical University, No. 23 Pingjiang Road, Hexi District, Tianjin, 300211.)
Abstract	The aim of the present study was to investigate the effect of HIF1 on Foxp3 expression in CD4(+) CD25(-) T lymphocytes. CD4(+) CD25(-) T lymphocytes were sorted from PBMC using a CD4(+) CD25(+) regulatory T cell isolation kit. Lentivirus containing lentiviral vector that overexpressed HIF1 (HIF-lenti) and those containing empty expression vector (control-lenti) were produced. Meanwhile, lentivirus that contained lentiviral vector that suppressed HIF1 expression (siHIF-lenti) and those containing control vector (sicontrol-lenti) were also generated. The sorted CD4(+) CD25(-) T lymphocytes were infected with HIF-lenti, control-lenti, siHIF-lenti, and sicontrol-lenti, respectively. Approximately 72 hr after transduction, real-time PCR and Western blot were carried out to analyze the RNA and protein expression level of HIF1 and Foxp3. CD4(+) CD25(-) T lymphocytes cultured under 21% O2 , 5% CO2 (normoxia) and 1% O2 , 5% CO2 (hypoxia) were used as control. Our results showed that overexpression of HIF1 increased both mRNA and protein expression of Foxp3 and, meanwhile, suppression of HIF1 expression by RNAi could reverse high Foxp3 expression in CD4(+) CD25(-) T lymphocytes caused by hypoxic culture. These results suggested that hypoxia could stimulate Foxp3 expression by increasing HIF1 expression in CD4(+) T lymphocytes which may promote CD4(+) T lymphocytes to convert to Treg.
File Format	HTM / HTML
ISSN	03855600
Issue Number	7
Volume Number	58
e-ISSN	13480421
Journal	Microbiology and Immunology
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2014-07-01
Publisher Place	Australia
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Immunology Cd4-positive T-lymphocytes Metabolism Forkhead Transcription Factors Genetics Gene Expression Hypoxia-inducible Factor 1, Alpha Subunit T-lymphocyte Subsets Immunology Cell Line Humans Anoxia Interleukin-2 Receptor Alpha Subunit Neoplasms Rna, Messenger T-lymphocytes, Regulatory Journal Article
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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