NDLI: In vitro antileishmanial activity of methanolic and aqueous extracts of Eucalyptus camaldulensis against Leishmania major.

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In vitro antileishmanial activity of methanolic and aqueous extracts of Eucalyptus camaldulensis against Leishmania major.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nosratabadi, Seyyed Jafar Sharifi, Iraj Sharififar, Fariba Bamorovat, Mehdi Daneshvar, Hamid Mirzaie, Mohammad
Description	Country affiliation: Iran Author Affiliation: Nosratabadi SJ ( Islamic Azad University, Kerman Branch, Kerman, Iran.); Sharifi I ( Leishmaniasis Research Center, Kerman University of Medical Sciences, 76169-14115 Kerman, Iran.); Sharififar F ( Faculty of Pharmacy, Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.); Bamorovat M ( Leishmaniasis Research Center, Kerman University of Medical Sciences, 76169-14115 Kerman, Iran.); Daneshvar H ( Immunology Department, Kerman University of Medical Sciences, Kerman, Iran.); Mirzaie M ( Pathobiology Department, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.)
Abstract	Pentavalent antimony compounds are expensive, toxic and drug resistance is prevalent, whereas the plant extract derivatives are safe. In the present study, the effect of methanolic and aqueous extracts of Eucalyptus camaldulensis on the promastigotes of Leishmania major was evaluated. The methanolic and aqueous extracts of E. camaldulensis leaves were prepared. The compounds were dried and powdered. Serial dilutions of the extracts and control drugs in phosphate buffer solution were prepared. The stationary phase promastigotes of L. major were incubated to the methanolic and aqueous extractions in vitro. Tartar emetic was used as the positive control drug. After 72 h of incubation the activity of the extracts was measured, using MTT method. The IC50 values (50 % inhibitory concentration) were 586.2 ± 47.6 and 1,108.6 ± 51.9 µg/ml for methanolic and aqueous extracts, respectively, whereas it was 32.5 ± 6.8 µg/ml for tartar emetic. The results indicated that the methanolic extract was more effective than aqueous extract, although there was no significant difference. The extracts were less effective as compared to the control drug. Further investigation is required to evaluate these extracts on clinical stage in macrophage-amastigote model.
File Format	HTM / HTML
ISSN	09717196
e-ISSN	09750703
DOI	10.1007/s12639-013-0377-3
Journal	Journal of Parasitic Diseases
Issue Number	1
Volume Number	39
Language	English
Publisher	Springer
Publisher Date	2015-03-01
Publisher Place	India
Access Restriction	Open
Subject Keyword	Discipline Parsitology
Content Type	Text
Resource Type	Article
Subject	Parasitology

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In vitro antileishmanial activity of methanolic and aqueous extracts of Eucalyptus camaldulensis against Leishmania major

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