NDLI: First identification of Sarcocystis hominis in Iranian traditional hamburger.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ahmadi, M. Moghaddam Hajimohammadi, B. Eslami, G. Oryan, A. Yasini Ardakani, S. A. Zohourtabar, A. Zare, S.
Description	Country affiliation: Iran Author Affiliation: Ahmadi MM ( Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.); Hajimohammadi B ( Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.); Eslami G ( Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.); Oryan A ( Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.); Yasini Ardakani SA ( Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Yazd, Iran.); Zohourtabar A ( Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.); Zare S ( Yazd Health Office, Yazd, Iran.)
Abstract	Zoonotic concerns of cattle sarcocystosis are of importance, because humans are the final host for Sarcocystis hominis. Therefore the meat products containing beef may encompass sarcocysts which endanger food safety. In this study, we described the first report of molecular identification of S. hominis in Iranian traditional hamburgers using PCR-RFLP. Throughout a pilot research that was carried out to setup a molecular approach to identify the Sarcocystis spp., using PCR-RFLP, a sample of raw Iranian traditional hamburger was purchased from a street food seller located in Yazd, central Iran in May 2013. DNA extraction was done, by salting out method; briefly, the sample was lysed with NET buffer. The DNA purification and precipitation was then performed. Amplicon and digestion results were analyzed, using gel agarose electrophoresis. The results showed a PCR product with 926 bp in length after amplification and 376 and 550 bp in length after digestion. This product was identified as S. hominis. To the best of our knowledge, this is the first report of S. hominis infection in Iranian hamburger.
File Format	HTM / HTML
ISSN	09717196
Issue Number	4
Journal	Journal of Parasitic Diseases
Volume Number	39
e-ISSN	09750703
Language	English
Publisher	Springer
Publisher Date	2015-12-01
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Parsitology
Content Type	Text
Resource Type	Article
Subject	Parasitology

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