NDLI: Force volume and stiffness tomography investigation on the dynamics of stiff material under bacterial membranes.

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Force volume and stiffness tomography investigation on the dynamics of stiff material under bacterial membranes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Longo, Giovanni Rio, Laura Marques Roduit, Charles Trampuz, Andrej Bizzini, Alain Dietler, Giovanni Kasas, Sandor
Description	Country affiliation: Switzerland Author Affiliation: Longo G ( Laboratory of Physics of Living Matter, EPFL, Lausanne, Switzerland. giovanni.longo@epfl.ch)
Abstract	The determination of the characteristics of micro-organisms in clinical specimens is essential for the rapid diagnosis and treatment of infections. A thorough investigation of the nanoscale properties of bacteria can prove to be a fundamental tool. Indeed, in the latest years, the importance of high resolution analysis of the properties of microbial cell surfaces has been increasingly recognized. Among the techniques available to observe at high resolution specific properties of microscopic samples, the Atomic Force Microscope (AFM) is the most widely used instrument capable to perform morphological and mechanical characterizations of living biological systems. Indeed, AFM can routinely study single cells in physiological conditions and can determine their mechanical properties with a nanometric resolution. Such analyses, coupled with high resolution investigation of their morphological properties, are increasingly used to characterize the state of single cells. In this work, we exploit the capabilities and peculiarities of AFM to analyze the mechanical properties of Escherichia coli in order to evidence with a high spatial resolution the mechanical properties of its structure. In particular, we will show that the bacterial membrane is not mechanically uniform, but contains stiffer areas. The force volume investigations presented in this work evidence for the first time the presence and dynamics of such structures. Such information is also coupled with a novel stiffness tomography technique, suggesting the presence of stiffer structures present underneath the membrane layer that could be associated with bacterial nucleoids.
File Format	HTM / HTML
ISSN	09523499
Issue Number	5
Journal	Journal of Molecular Recognition
Volume Number	25
e-ISSN	10991352
Language	English
Publisher	Wiley
Publisher Date	2012-05-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Molecular Biology Cell Membrane Ultrastructure Elasticity Escherichia Coli Microscopy, Atomic Force Tomography, X-ray Computed Cytology Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Structural Biology Molecular Biology

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