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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chung, Ji W. Shin, Dongjin Kwak, June M. Seog, Joonil |
| Description | Country affiliation: United States Author Affiliation: Chung JW ( Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.) |
| Abstract | The selective interactions between DNA and miniature (39 residues) engineered peptide were directly measured at the single-molecule level by using atomic force microscopy. This peptide (p007) contains an -helical recognition site similar to leucine zipper GCN4 and specifically recognizes the ATGAC sequence in the DNA with nanomolar affinity. The average rupture force was 42.1 pN, which is similar to the unbinding forces of the digoxigenin-antidigoxigenin complex, one of the strongest interactions in biological systems. The single linear fit of the rupture forces versus the logarithm of pulling rates showed a single energy barrier with a transition state located at 0.74 nm from the bound state. The smaller koff compared with that of other similar systems was presumably due to the increased stability of the helical structure by putative folding residues in p007. This strong sequence-specific DNA-peptide interaction has a potential to be utilized to prepare well-defined mechanically stable DNA-protein hybrid nanostructures. |
| File Format | HTM / HTML |
| ISSN | 09523499 |
| Issue Number | 6 |
| Volume Number | 26 |
| e-ISSN | 10991352 |
| Journal | Journal of Molecular Recognition |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2013-06-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Molecular Biology Dna Chemistry Hydrophobic And Hydrophilic Interactions Microscopy, Atomic Force Methods Peptides Amino Acid Sequence Humans Models, Biological Molecular Weight Nanostructures Protein Binding Physiology Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology |
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