NDLI: Isocyanates induces DNA damage, apoptosis, oxidative stress, and inflammation in cultured human lymphocytes.

Please wait, while we are loading the content...

Year: 2016 Volume: 30

Year: 2015 Volume: 29

Year: 2014 Volume: 28

Year: 2013 Volume: 27

Year: 2012 Volume: 26

Year: 2011 Volume: 25

Year: 2010 Volume: 24

Year: 2009 Volume: 23

Year: 2008 Volume: 22

Year: 2008 Volume: 22 Issue: 6

Diethyl maleate-induced oxidative stress leads to testicular germ cell apoptosis involving Bax and Bcl-2.

Piperine activates testicular apoptosis in adult rats.

N-Nitrosodimethylamine changes the expression of glutathione S-transferase in the liver of male mice: The role of antioxidants.

Novel indoloquinoline derivative, IQDMA, inhibits STAT5 signaling associated with apoptosis in K562 cells.

Purification and characterization of a novel high molecular weight exotoxin produced by red tide phytoplankton, Alexandrium tamarense.

The role of nuclear factor-kappaB in rats of radiocontrast-media-induced nephropathy.

Segment-specific proximal tubule injury in tripterygium glycosides intoxicated rats.

Isocyanates induces DNA damage, apoptosis, oxidative stress, and inflammation in cultured human lymphocytes.

Year: 2008 Volume: 22 Issue: 5

Year: 2008 Volume: 22 Issue: 4

Year: 2008 Volume: 22 Issue: 3

Year: 2008 Volume: 22 Issue: 2

Year: 2008 Volume: 22 Issue: 1

Year: 2007 Volume: 21

Year: 2006 Volume: 20

Year: 2005 Volume: 19

Isocyanates induces DNA damage, apoptosis, oxidative stress, and inflammation in cultured human lymphocytes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mishra, Pradyumna Kumar Panwar, Hariom Bhargava, Arpit Gorantla, Venkata Raghuram Jain, Subodh Kumar Banerjee, Smita Maudar, Kewal Krishan
Description	Author Affiliation: Mishra PK ( Bhopal Memorial Hospital & Research Centre, Raisen Bypass Road, Bhopal 462 038, India. pkm 8bh@yahoo.co.uk)
Abstract	Isocyanates, a group of low molecular weight aromatic and aliphatic compounds containing the isocyanate group (-NCO), are important raw materials with diverse industrial applications; however, pathophysiological implications resulting from occupational and accidental exposures of these compounds are hitherto unknown. Although preliminary evidence available in the literature suggests that isocyanates and their derivatives may have deleterious health effects including immunotoxicity, but molecular mechanisms underlying such an effect have never been addressed. The present study was carried out to assess the immunotoxic response of methyl isocyanate (MIC) on cultured human lymphocytes isolated from healthy human volunteers. Studies were conducted to evaluate both dose-dependent and time-course response (n = 3), using N-succinimidyl N-methylcarbamate, a surrogate chemical substitute to MIC. Evaluation of DNA damage by ataxia telangiectasia mutated (ATM) and gamma H2AX protein phosphorylation states; measure of apoptotic index through annexin-V/PI assay, apoptotic DNA ladder assay, and mitochondrial depolarization; induction of oxidative stress by CM-H2DCFDA and formation of 8-hydroxy-2' deoxy guanosine; levels of antioxidant defense system enzyme glutathione reductase; and multiplex cytometric bead array analysis to quantify the secreted levels of inflammatory cytokines, interleukin-8, interleukin-1beta, interleukin-6, interleukin-10, tumor necrosis factor, and interleukin-12p70 parameters were carried out. The results of the study showed a dose- and time-dependent response, providing evidence to hitherto unknown molecular mechanisms of immunotoxic consequences of isocyanate exposure at a genomic level. We anticipate these data along with other studies reported in the literature would help to design better approaches in risk assessment of occupational and accidental exposure to isocyanates.
File Format	HTM / HTML
ISSN	10956670
Issue Number	6
Volume Number	22
e-ISSN	10990461
Journal	Journal of Biochemical and Molecular Toxicology
Language	English
Publisher	Wiley
Publisher Date	2008-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Discipline Toxicology Apoptosis Drug Effects Dna Damage Inflammation Pathology Isocyanates Pharmacology Lymphocytes Oxidative Stress Annexin A5 Metabolism Antioxidants Ataxia Telangiectasia Mutated Proteins Cell Cycle Proteins Cells, Cultured Cytokines Dna Fragmentation Dna-binding Proteins Deoxyguanosine Analogs & Derivatives Enzyme-linked Immunosorbent Assay Fluorescein-5-isothiocyanate Glutathione Reductase Histones Humans Inflammation Mediators Phosphorylation Propidium Protein-serine-threonine Kinases Reactive Oxygen Species Tumor Suppressor Proteins Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Molecular Biology Biochemistry Toxicology Molecular Medicine

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in