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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yuan, Dong Shen, Zhonglan Liu, Rutao Chi, Zhenxing Zhu, Jianhua |
| Description | Author Affiliation: Yuan D ( Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, Jinan 250100, People's Republic of China.) |
| Abstract | The interaction of Ce(3+) to bovine serum albumin (BSA) has been investigated mainly by fluorescence spectra, UV-vis absorption spectra, and circular dichroism (CD) under simulative physiological conditions. Fluorescence data revealed that the quenching mechanism of BSA by Ce(3+) was a static quenching process, the binding constant is 6.70 × 10(5) , and the number of binding site is 1. The thermodynamic parameters (ΔH = -29.94 kJ mol(-1) , ΔG = -32.38 kJ mol(-1) , and ΔS = 8.05 J mol(-1) K(-1) ) indicate that electrostatic effect between the protein and the Ce(3+) is the main binding force. In addition, UV-vis, CD, and synchronous fluorescence results showed that the addition of Ce(3+) changed the conformation of BSA. |
| File Format | HTM / HTML |
| ISSN | 10956670 |
| Issue Number | 4 |
| Volume Number | 25 |
| e-ISSN | 10990461 |
| Journal | Journal of Biochemical and Molecular Toxicology |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2011-07-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Molecular Biology Discipline Biochemistry Discipline Toxicology Cerium Chemistry Serum Albumin, Bovine Animals Cattle Circular Dichroism Hydrogen Bonding Protein Binding Protein Structure, Secondary Spectrometry, Fluorescence Spectrophotometry Thermodynamics Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health, Toxicology and Mutagenesis Medicine Molecular Biology Biochemistry Toxicology Molecular Medicine |
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