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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lee, S. E. Bae, S. J. Kim, S. Y. Kim, Y. R. Rhee, J. H. |
| Description | Responsible library: WPRO |
| Abstract | Recently we have shown that a bacterial flagellin, Vibrio vulnifiucs FlaB (Vv-FlaB), has a strong adjuvant activity to induce protective immune response. In order to investigate the adjuvanticity of Vv-FlaB, we prepared highly purified recombinant protein by using an intein fusion protein purification system. However, in the process of the purification, we unexpectedly encountered a contamination with a 70 kDa protein. We proved the 70 kDa protein as the heat shock protein 70 (HSP70) by Western blotting. Unfortunately, it was reported that the HSP70 has a strong adjuvanticity. In this study we investigated the role of contaminating HSP70 on the Vv-FlaB-mediated adjuvanticity. We separated Vv-FlaB and HSP70 by using a high performance protein purification chromatography and compared adjuvant activities of Vv-FlaB, HSP70 and Vv-FlaB/HSP70 mixture. Using an intranasal immunization mouse model, we observed that co-administration of the flagellin with tetanus toxoid (TT) induced significantly enhanced TT-specific antibody (Ig) responses. However contaminating doses of HSP70 did not affect the adjuvanticity of Vv-FlaB and furthermore HSP70 alone did not enhance TT-specific Ig response and protective immunity against lethal challenge with tetanus toxin. These results show that the HSP70 contaminating Vv-FlaB preparations did not affect the adjuvanticity of Vv-FlaB. |
| File Format | HTM / HTML |
| ISSN | 15982467 |
| e-ISSN | 20930429 |
| Journal | Journal of Bacteriology and Virology |
| Issue Number | 4 |
| Volume Number | 35 |
| Language | Korean |
| Publisher | Korean Society for Microbiology Korean Society of Virology |
| Publisher Date | 2005-01-01 |
| Publisher Place | Korea (South) |
| Access Restriction | Open |
| Subject Keyword | Discipline Microbiology Discipline Virology Discipline Infectious Diseases Discipline Bacteriology Hsp70 Heat-shock Proteins Flagellin Thrombin Time Assay Vibrio Protein Purification |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Immunology Microbiology |
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