NDLI: Exploitation of Culture Medium for Mycobacterium tuberculosis

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Exploitation of Culture Medium for Mycobacterium tuberculosis

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Exploitation of Culture Medium for Mycobacterium tuberculosis

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Hyejin Ryoo, Sungweon
Description	Responsible library: WPRO
Abstract	The culture media for Mycobacterium tuberculosis (MTB) were contrived from both egg-based and agar based ingredients. In 1903, Dorset introduced the first egg-based medium. It was followed by the invention of Lowenstein-Jensen and Ogawa media that contain whole eggs as nutrient and malachite green to inhibit the growth of contaminants. These media have been used worldwide for their usefulness and inexpensiveness. However they have a fundamental disadvantage that the cultivation time for mycobacterial growth takes more than 4 weeks. In 1947, Dubos introduced the first agar medium and followed by the invention of the 7H10 by Middlebrook and Cohn. A powder base of these media contains agar, combination of seven salts, L-glutamic acid, pyridoxine, biotin, and malachite green. This requires the addition of the oleic acid, albumin, dextrose, and catalase (OADC) growth supplement. BACTEC MGIT960 has recently been introduced for rapid cultivation of MTB, which is fully automated liquid culture system with modified 7H9 broth. Agar-based medium developed by Middlebrook has a number of advantages over egg-based medium. One of them is transparency, which enables earlier detection of growing colonies. The major disadvantages of agar media are the high cost of OADC and the need for a CO2 incubator. In conclusion, there is a need for a new agar medium, which can be produced at a lower cost and earlier growth detection. In this review, we introduced the growth promoting factors which can be used as an alternative new growth supplement, cAMP and resuscitation-promoting factor (Rpf). These factors may abrogate a lag in adaption of the bacilli in culture media.
File Format	HTM / HTML
ISSN	15982467
e-ISSN	20930429
Journal	Journal of Bacteriology and Virology
Issue Number	4
Volume Number	41
Language	Korean
Publisher	Korean Society for Microbiology Korean Society of Virology
Publisher Date	2011-01-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Virology Discipline Infectious Diseases Discipline Bacteriology
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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