NDLI: Non-contact laser-scanning confocal microscopy of the human cornea in vivo.

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Non-contact laser-scanning confocal microscopy of the human cornea in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pritchard, Nicola Edwards, Katie Efron, Nathan
Description	Country affiliation: Australia Author Affiliation: Pritchard N ( Institute of Health and Biomedical Innovation, and School of Optometry and Vision Science, Queensland University of Technology, Kelvin Grove, Queensland, Australia.); Edwards K ( Institute of Health and Biomedical Innovation, and School of Optometry and Vision Science, Queensland University of Technology, Kelvin Grove, Queensland, Australia.); Efron N ( Institute of Health and Biomedical Innovation, and School of Optometry and Vision Science, Queensland University of Technology, Kelvin Grove, Queensland, Australia. Electronic address: n.efron@qut.edu.au.)
Abstract	PURPOSE: To investigate the utility of using non-contact laser-scanning confocal microscopy (NC-LSCM), compared with the more conventional contact laser-scanning confocal microscopy (C-LSCM), for examining corneal substructures in vivo. METHODS: An attempt was made to capture representative images from the tear film and all layers of the cornea of a healthy, 35 year old female, using both NC-LSCM and C-LSCM, on separate days. RESULTS: Using NC-LSCM, good quality images were obtained of the tear film, stroma, and a section of endothelium, but the corneal depth of the images of these various substructures could not be ascertained. Using C-LSCM, good quality, full-field images were obtained of the epithelium, subbasal nerve plexus, stroma, and endothelium, and the corneal depth of each of the captured images could be ascertained. CONCLUSIONS: NC-LSCM may find general use for clinical examination of the tear film, stroma and endothelium, with the caveat that the depth of stromal images cannot be determined when using this technique. This technique also facilitates image capture of oblique sections of multiple corneal layers. The inability to clearly and consistently image thin corneal substructures - such as the tear film, subbasal nerve plexus and endothelium - is a key limitation of NC-LSCM.
File Format	HTM / HTML
ISSN	13670484
Issue Number	1
Volume Number	37
e-ISSN	14765411
Journal	Contact Lens and Anterior Eye
Language	English
Publisher	Elsevier
Publisher Date	2014-02-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Ophthalmology Cornea Cytology Microscopy, Confocal Methods Adult Female Healthy Volunteers Humans Instrumentation Pilot Projects Tears Chemistry Journal Article
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Optometry

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