NDLI: Cytomegalovirus induces stage-dependent enamel defects and misexpression of amelogenin, enamelin and dentin sialophosphoprotein in developing mouse molars.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jaskoll, Tina Abichaker, George Htet, Khine Bringas, Pablo Morita, Scott Sedghizadeh, Parish P. Melnick, Michael
Description	Country affiliation: United States Author Affiliation: Jaskoll T ( Laboratory for Developmental Genetics, University of Southern California, Los Angeles, CA 90089-0641, USA. tjaskoll@usc.edu)
Abstract	Of the approximately 8,400 children born each year in the US with cytomegalovirus (CMV)-induced birth defects, more than one third exhibit hypoplasia and hypocalcification of tooth enamel. Our prior studies indicated that CMV severely delayed, but did not completely interrupt, early mouse mandibular first molar morphogenesis in vitro. The aim of the present study was to examine the effects of CMV infection on progressive tooth differentiation and amelogenesis. Since initial CMV infection in human fetuses can occur at different developmental times, we varied the stage of initial viral infection (that is, Cap stage, Early Bell stage and Bell stage), as well as the duration of infection. CMV infection of embryonic mouse mandibular first molars in vitro induces tooth dysmorphogenesis and enamel defects in a developmental stage- and duration-dependent manner. Cap stage- and Early Bell stage-infected molars exhibit enamel agenesis and Bell stage-infected molars exhibit enamel hypoplasia. This viral-induced pathology is coincident with stage-dependent changes in Amelx, Enam and Dspp gene expression, distribution of amelogenin, enamelin and DSP proteins, cell proliferation localization and dedifferentiation of secretory ameloblasts. Importantly, our data indicate that specific levels of Amelx and Dspp gene expression define whether mouse CMV induces enamel agenesis or hypoplasia.
File Format	HTM / HTML
ISSN	14226405
Issue Number	4
Volume Number	192
e-ISSN	14226421
Journal	Cells Tissues Organs
Language	English
Publisher	Karger
Publisher Date	2010-01-01
Publisher Place	Switzerland
Access Restriction	Subscribed
Subject Keyword	Discipline Cell Biology Discipline Anatomy Amelogenesis Amelogenin Genetics Cytomegalovirus Pathogenicity Dental Enamel Abnormalities Molar Embryology Virology Ameloblasts Cytology Amelogenesis Imperfecta Biosynthesis Animals Cell Dedifferentiation Cell Differentiation Cell Proliferation Dental Enamel Hypoplasia Dental Enamel Proteins Extracellular Matrix Proteins Gene Expression Gene Expression Profiling Mice Physiology Odontoblasts Phosphoproteins Sialoglycoproteins Tissue Culture Techniques Journal Article
Content Type	Text
Resource Type	Article
Subject	Anatomy Histology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in