NDLI: Value of immunohistochemistry in the detection of BRAF(V600E) mutations in fine-needle aspiration biopsies of papillary thyroid carcinoma.

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Value of immunohistochemistry in the detection of BRAF(V600E) mutations in fine-needle aspiration biopsies of papillary thyroid carcinoma.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zimmermann, Anne-Katrin Camenisch, Ulrike Rechsteiner, Markus P. Bode-Lesniewska, Beata Rössle, Matthias
Description	Country affiliation: Switzerland Author Affiliation: Zimmermann AK ( Institute of Surgical Pathology, University Hospital, Zurich, Switzerland.)
Abstract	BACKGROUND: Fine-needle aspiration biopsy (FNAB) is important in the diagnostic establishment of suspicious thyroid nodules. In thyroid neoplasms, mutation of the BRAF gene occurs rather exclusively in papillary thyroid carcinoma (PTC) and results in>98% of the cases in V600E amino acid substitution. In the current study, the authors investigated the diagnostic value of a recently described monoclonal antibody that detects this specific mutation on FNAB specimens from patients with PTC. METHODS: BRAF(V600E) status of FNAB cell blocks from 55 patients with PTC was analyzed by immunohistochemistry (IHC) with the new BRAF(V600E) antibody (clone VE1) and by Sanger sequencing (SaS). In discrepant cases, ultra-deep sequencing was also performed. Available corresponding histological specimens were investigated by IHC and, in selected cases, with SaS as well. RESULTS: All cases yielded evaluable IHC staining results of the cell block sections with good interobserver agreement (kappa value, 0.650). Ten tumors (18.2%) demonstrated no staining, 10 tumors (18.2%) demonstrated equivocal staining, 25 tumors (45.4%) demonstrated moderate staining, and 10 tumors (18.2%) demonstrated strong staining. SaS was able to be performed in 48 cases. Nineteen cases demonstrated wild-type BRAF and 29 cases were found to have the BRAF(V600E) mutation. After performing ultra-deep sequencing 1 false-positive and 2 false-negative VE1 IHC cases remained, resulting in a sensitivity of 93.8% and a specificity of 93.8%. CONCLUSIONS: BRAF(V600E) mutations in FNAB specimens from patients with PTC can be reliably detected in most cases by IHC with a new mutation-specific antibody. Interpretation of VE1 IHC staining results on cell block slides of PTC can be difficult in some cases.
File Format	HTM / HTML
ISSN	1934662X
Issue Number	1
Volume Number	122
e-ISSN	19346638
Journal	Cancer Cytopathology
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2014-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Cytology Discipline Pathology Discipline Oncology Carcinoma Genetics Pathology Proto-oncogene Proteins B-raf Thyroid Neoplasms Adult Aged Aged, 80 And Over Biopsy, Fine-needle Methods Surgery Chi-square Distribution Cohort Studies Confidence Intervals Female Gene Expression Regulation, Neoplastic High-throughput Nucleotide Sequencing Humans Immunohistochemistry Male Middle Aged Mutation Reproducibility Of Results Retrospective Studies Statistics, Nonparametric Evaluation Studies Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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