NDLI: Tectonic 1 Is a Key Regulator of Cell Proliferation in Pancreatic Cancer.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhao, Shiyong Chen, Xuedong Wan, Ming Jiang, Xingming Li, Chunlong Cui, Yunfu Kang, Pengcheng
Description	Country affiliation: China Author Affiliation: Zhao S ( 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .); Chen X ( 2 Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University , Harbin, China .); Wan M ( 3 Harbin Medical University , Harbin, China .); Jiang X ( 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .); Li C ( 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .); Cui Y ( 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .); Kang P ( 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .)
Abstract	Pancreatic cancer is notoriously becoming one of the most devastating human cancers leading to death. However, clinical challenges still remain in diagnosis and treatment of this ticklish cancer. In the present study, the authors identified a new gene, Tectonic 1 (TCTN1), as a key regulator of cell proliferation in pancreatic cancer. Lentivirus-mediated short hairpin RNA (shRNA) was employed to knock down endogenous TCTN1 expression in PANC-1 pancreatic cancer cells. Knockdown of TCTN1 expression potently inhibited cell viability and proliferation, as determined by MTT and colony formation assays. Western blotting analysis also showed that knockdown of TCTN1 suppressed the expression of cdc2, while it induced that of p21 and p27. Flow cytometry analysis showed that depletion of TCTN1 in PANC-1 cells led to cell cycle arrest in the G2/M phase as well as apoptosis. Besides, depletion of TCTN1 led to the increase of Bax and cleavage of PARP-1, but the decrease of bcl2 by western blotting. The data indicate that TCTN1 is indispensable for pancreatic cancer cell proliferation, which provides a novel alternative to targeted therapy of pancreatic cancer and deserves further investigation.
File Format	HTM / HTML
ISSN	10849785
Issue Number	1
Volume Number	31
e-ISSN	15578852
Journal	Cancer Biotherapy & Radiopharmaceuticals
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2016-02-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Pharmacology Discipline Oncology Cell Proliferation Membrane Proteins Metabolism Pancreatic Neoplasms Pathology Apoptosis Blotting, Western Cell Cycle Flow Cytometry Humans Immunoenzyme Techniques Lentivirus Genetics Antagonists & Inhibitors Rna, Messenger Rna, Small Interfering Real-time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Radiology, Nuclear Medicine and Imaging Cancer Research Pharmacology Oncology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in