NDLI: Prion protein polymorphisms and estimation of risk of scrapie in East Asian sheep.

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Prion protein polymorphisms and estimation of risk of scrapie in East Asian sheep.

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Prion protein polymorphisms and estimation of risk of scrapie in East Asian sheep.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tsunoda, Kenji Namikawa, Takao Sato, Keizo Hasnath, M. A. Nyunt, Maung Maung Rajbandary, Heramba B. Loc, Chau Ba Zanchiv, Ts Chang, Hong Sun, Wei Dorji, Tasi
Spatial Coverage	Europe Far East
Description	Country affiliation: Japan Author Affiliation: Tsunoda K ( Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan. tsunoken@med.showa-u.ac.jp)
Abstract	Allele and genotype frequency distributions of prion protein (PrP) polymorphisms at three codons, 136, 154, and 171, in East Asian sheep were determined by PCR-RFLP analysis using 553 animals from nine local breeds of the northern group and four local breeds of the southern group. Based on the genotype distribution, the risk score for scrapie was estimated. Among the local breeds, ARQ appeared predominantly (0.7701-1), followed by ARH and ARR. From such a biased allele distribution, it was difficult to ascertain the prevalent genetic relationships. A marked difference in allele frequencies between the northern and southern groups was seen (P < 0.0001). The East Asian sheep had ARQ at the highest frequency (0.8834); in European sheep it was 0.5317. According to an assessment of scrapie risk in the PrP genotype classes, the predominant ARQ/ARQ in East Asian sheep corresponded to the risk score of R4. This finding suggests that East Asian sheep have a high level of genetic susceptibility to scrapie.
File Format	HTM / HTML
ISSN	00062928
Issue Number	1-2
Volume Number	48
e-ISSN	15734927
Journal	Biochemical Genetics
Language	English
Publisher	Springer
Publisher Date	2010-02-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Molecular Biology Polymorphism, Genetic Prions Genetics Scrapie Epidemiology Sheep Diseases Animals Codon Dna Primers Europe Far East Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sheep Journal Article
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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