NDLI: Cultivation and molecular identification of Ehrlichia canis and Ehrlichia chaffeensis from a naturally co-infected dog in Venezuela.

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Cultivation and molecular identification of Ehrlichia canis and Ehrlichia chaffeensis from a naturally co-infected dog in Venezuela.

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Cultivation and molecular identification of Ehrlichia canis and Ehrlichia chaffeensis from a naturally co-infected dog in Venezuela.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gutiérrez, Clara N. Martínez, María Sánchez, Erlinda De Vera, Marisol Rojas, Marìa Ruiz, Joahnny Triana-Alonso, Francisco J.
Spatial Coverage	Venezuela
Description	Country affiliation: Venezuela Author Affiliation: Gutiérrez CN ( Biomedical Research Institute (BIOMED-UC), Univresity of Carabobo campus Aragua, Maracay, Venezuela.)
Abstract	BACKGROUND: Diagnosis of canine ehrlichiosis in Venezuela is normally performed by examination of buffy coat smears (BCS). Characteristic inclusion bodies are frequently observed in leukocytes and platelets from dogs with clinical signs of the disease. OBJECTIVE: The purpose of this study was to investigate the co-infection of a dog with Ehrlichia canis and E hrlichia chaffeensis using microbiological and molecular techniques. METHODS: Primary cultures of monocytes from a dog showing signs of ehrlichiosis were performed. Ehrlichial inclusions in blood cells were demonstrated by BCS and in cultured cell smears with direct immunofluorescence and Dip Quick staining. Nested PCR analysis was performed with DNA from blood samples and cultures, using primers specific for E. canis and E. chaffeensis. The amplified DNA fragments were sequenced to confirm the specificity of the amplifications. RESULTS: The BCS of the naturally infected dog contained intracellular morulae. Ehrlichial inclusions were observed 9 days after inoculation of the primary cultures. After 3 passages with monocytes from a healthy dog, 65% of infected cells, and cells with >60 morulae were observed. A healthy female German Shepherd dog, seronegative for E. canis and E. chaffeensis antigens and without contact to ticks, was inoculated with an infected culture. The animal developed signs of canine monocytic ehrlichiosis and became seropositive. Nested PCR results and sequencing of amplified DNA fragments demonstrated the simultaneous presence of E. canis and E. chaffeensis in both dogs. CONCLUSIONS: This is the first report of E. chaffeensis in dogs in South America. This organism was previously identified in dogs by PCR only in the United States.
File Format	HTM / HTML
ISSN	02756382
Issue Number	3
Volume Number	37
e-ISSN	1939165X
Journal	Veterinary Clinical Pathology
Language	English
Publisher	Wiley
Publisher Date	2008-09-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Discipline Pathology Dog Diseases Microbiology Ehrlichia Canis Isolation & Purification Ehrlichia Chaffeensis Ehrlichiosis Veterinary Animals Dna, Bacterial Genetics Dogs Classification Female Monocytes Venezuela Case Reports Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Case study Article
Subject	Veterinary Medicine

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