NDLI: Freeze-thaw cycles enhance decellularization of large tendons.

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Freeze-thaw cycles enhance decellularization of large tendons.

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Freeze-thaw cycles enhance decellularization of large tendons.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Burk, Janina Erbe, Ina Berner, Dagmar Kacza, Johannes Kasper, Cornelia Pfeiffer, Bastian Winter, Karsten Brehm, Walter
Description	Country affiliation: Germany Author Affiliation: Burk J ( 1 Translational Centre for Regenerative Medicine, University of Leipzig , Germany .)
Abstract	The use of decellularized tendon tissue as a scaffold for tendon tissue engineering provides great opportunities for future clinical and current research applications. The aim of this study was to assess the effect of repetitive freeze-thaw cycles and two different detergents, t-octyl-phenoxypolyethoxyethanol (Triton X-100) and sodium dodecyl sulfate (SDS), on decellularization effectiveness and cytocompatibility in large tendons. Freshly collected equine superficial and deep digital flexor tendons were subjected to decellularization according to four different protocols (1 and 2: freeze-thaw cycles combined with either Triton X-100 or SDS; 3 and 4: Triton X-100 or SDS). Decellularization effectiveness was assessed based on the reduction of vital cell counts, histologically visible nuclei, and DNA content. Transmission electron microscopy was performed to evaluate cellular and extracellular matrix integrity. Further, cytocompatibility of scaffolds that had been decellularized according to the protocols including freeze-thaw cycles (protocols 1 and 2) was assessed by seeding the scaffolds with superparamagnetic iron oxide labeled mesenchymal stromal cells and monitoring the cells histologically and by magnetic resonance imaging for two weeks. Decellularization was significantly more effective when using the protocols including freeze-thaw cycles, leaving only roughly 1% residual nuclei and 20% residual DNA, whereas samples that had not undergone additional freeze-thaw cycles contained roughly 20% residual nuclei and 40% residual DNA. No morphological extracellular matrix alterations due to decellularization could be observed. Scaffolds prepared by both protocols including freeze-thaw cycles were cytocompatible, but the cell distribution into the scaffold tended to be better in scaffolds that had been decellularized using freeze-thaw cycles combined with Triton X-100 instead of SDS.
File Format	HTM / HTML
ISSN	21524947
e-ISSN	21524955
DOI	10.1089/ten.TEC.2012.0760
Journal	Tissue Engineering Part C: Methods
Issue Number	4
Volume Number	20
Language	English
Publisher	Mary Ann Liebert
Publisher Date	2014-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biotechnology Freezing Tendons Cytology Tissue Engineering Animals Dextrans Chemistry Horses Magnetite Nanoparticles Mesenchymal Stromal Cells Staining And Labeling Ultrastructure Tissue Scaffolds Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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