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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ferreira, Elaine Giménez, Rosa Aguilera, Laura Guzmán, Karla Aguilar, Juan Badia, Josefa Baldomà, Laura |
| Description | Country affiliation: Spain Author Affiliation: Ferreira E ( Departament de Bioquímica i Biología Molecular, Facultat de Farmàcia, Institut de Biomedicina de la Universitat de Barcelona (IBUB), Universitat de Barcelona, Av. Diagonal, 643, E-08028 Barcelona, Spain. elainefmelo@gmail.com) |
| Abstract | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is considered a multifunctional protein with defined functions in numerous mammalian cellular processes. GAPDH functional diversity depends on various factors such as covalent modifications, subcellular localization, oligomeric state and intracellular concentration of substrates or ligands, as well as protein-protein interactions. In bacteria, alternative GAPDH functions have been associated with its extracellular location in pathogens or probiotics. In this study, new intracellular functions of Escherichia coli GAPDH were investigated following a proteomic approach aimed at identifying interacting partners using in vivo formaldehyde cross-linking followed by mass spectrometry. The identified proteins were involved in metabolic processes, protein synthesis and folding or DNA repair. Some interacting proteins were also identified in immunopurification experiments in the absence of cross-linking. Pull-down experiments and overlay immunoblotting were performed to further characterize the interaction with phosphoglycolate phosphatase (Gph). This enzyme is involved in the metabolism of 2-phosphoglycolate formed in the DNA repair of 3'-phosphoglycolate ends generated by bleomycin damage. We show that interaction between Gph and GAPDH increases in cells challenged with bleomycin, suggesting involvement of GAPDH in cellular processes linked to DNA repair mechanisms. |
| File Format | HTM / HTML |
| ISSN | 09232508 |
| Issue Number | 2 |
| Volume Number | 164 |
| e-ISSN | 17697123 |
| Journal | Research in Microbiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-02-01 |
| Publisher Place | France |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Escherichia Coli Proteins Metabolism Escherichia Coli Enzymology Glyceraldehyde-3-phosphate Dehydrogenases Protein Interaction Mapping Centrifugation Cross-linking Reagents Formaldehyde Immunoblotting Mass Spectrometry Protein Binding Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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