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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhu, Yanbing Wu, Liyun Chen, Yanhong Ni, Hui Xiao, Anfeng Cai, Huinong |
| Description | Author Affiliation: Zhu Y ( College of Food and Biological Engineering, Jimei University, Xiamen 361021, China); Wu L ( College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.); Chen Y ( College of Food and Biological Engineering, Jimei University, Xiamen 361021, China); Ni H ( College of Food and Biological Engineering, Jimei University, Xiamen 361021, China); Xiao A ( College of Food and Biological Engineering, Jimei University, Xiamen 361021, China); Cai H ( College of Food and Biological Engineering, Jimei University, Xiamen 361021, China) |
| Abstract | A novel alginate-degrading marine bacterium Microbulbifer sp. ALW1 was isolated from rotten brown alga. An extracellular alginate lyase was purified to electrophoretic homogeneity and had a molecular mass of about 26.0 kDa determined by SDS-PAGE and size exclusion chromatography. This enzyme showed activities towards both polyguluronate and polymannuronate indicating its bifunctionality while with preference for the former substrate. Using sodium alginate as a substrate, strain ALW1 alginate lyase was optimally active at 45 °C and pH 7.0. It was stable at 25 °C, 30 °C, 35 °C and 40 °C, but not stable at 50 °C. This alginate lyase showed good stability over a broad pH range (5.0-9.0). The enzyme activity was increased to 5.1 times by adding NaCl to a final concentration of 0.5M. Strain ALW1 alginate lyase produced disaccharide (majority) and trisaccharide from alginate indicating that this enzyme could be a good tool for preparation of alginate oligosaccharides with low degree of polymerization (DP). The alginate oligosaccharides displayed the scavenging abilities towards radicals (DPPH, ABTS(+) and hydroxyl) and the reducing power. Therefore, the hydrolysates exhibited the antioxidant activity and had potential as a natural antioxidant. |
| File Format | HTM / HTML |
| ISSN | 09445013 |
| Volume Number | 182 |
| e-ISSN | 16180623 |
| Journal | Microbiological Research |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Environmental Health Antioxidants Chemistry Bacterial Proteins Gammaproteobacteria Enzymology Isolation & Purification Polysaccharide-lyases Seawater Microbiology Alginates Metabolism Genetics Enzyme Stability Classification Glucuronic Acid Hexuronic Acids Hydrogen-ion Concentration Molecular Sequence Data Molecular Weight Phylogeny Substrate Specificity Temperature Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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