NDLI: Isolation of two cytochrome P450 forms, CYP2A19 and CYP1A, from pig liver microsomes.

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Isolation of two cytochrome P450 forms, CYP2A19 and CYP1A, from pig liver microsomes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Matal, J. Tunková, A. Siller, M. Anzenbacherová, E. Anzenbacher, P.
Description	Country affiliation: Czech Republic Author Affiliation: Matal J ( Department of Pharmacology, Palacky University, Olomouc 775-15, Czech Republic.)
Abstract	Cytochromes P450 comprise a superfamily of proteins that play a crucial role in the biotransformation of numerous chemicals. Purified CYPs can be used e.g. in studies on structure or determining the drug metabolism pathways. In this work, purification of the porcine CYP1A and CYP2A19 to electrophoretic homogeneity from the pig hepatic microsomes using octylamino Sepharose and hydroxylapatite column chromatography is reported. The proteins have been clearly recognized by commercial antibodies against rat and human CYP1A2 (porcine CYP1A) and human CYP2A6 (CYP2A19) respectively, using Western blot. Activities of both enzymes were determined by specific substrates, 7-ethoxyresorufin, 7-methoxyresorufin (CYP1A) and coumarin (CYP2A19). The isolated enzymes show kinetic parameters similar to human counterparts. Taken together, pig cytochromes can be used for the testing of veterinary drug metabolism, useful for the determination of drug residues in meat of pigs. The results obtained show that the pigs may be a suitable model for biotransformation of xenobiotics in humans.
File Format	HTM / HTML
ISSN	01407783
Issue Number	5
Volume Number	32
e-ISSN	13652885
Journal	Journal of Veterinary Pharmacology and Therapeutics
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2009-10-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Therapeutics Discipline Veterinary Medicine Discipline Pharmacology Aryl Hydrocarbon Hydroxylases Isolation & Purification Cytochrome P-450 Cyp1a1 Microsomes, Liver Enzymology Animals Blotting, Western Chromatography, Agarose Cytochrome P-450 Cyp2a6 Humans Swine Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Veterinary Pharmacology

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Isolation of two cytochrome P450 forms, CYP2A19 and CYP1A, from pig liver microsomes.

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