Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, Shan Lin, Hua Chen, Shijie Yang, Miao Yan, Qigui Wen, Caifang Hao, Zhongxiang Yan, Yubao Sun, Yingjie Hu, Juan Chen, Zhenrong Xi, Lixin |
| Spatial Coverage | China |
| Description | Author Affiliation: Zhao S ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Lin H ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Chen S ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Yang M ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Yan Q ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Wen C ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Hao Z ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Yan Y ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Sun Y ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Hu J ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Chen Z ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)); Xi L ( College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu)) |
| Abstract | Sensitive detection of Porcine circovirus-2 (PCV-2) is very important for surveillance of postweaning multisystemic wasting syndrome. Droplet digital polymerase chain reaction (ddPCR) is novel PCR method that can achieve high precision. Our study aimed to develop a sensitive assay utilizing ddPCR to detect PCV-2. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 25 copies/µL, a 4-fold greater sensitivity than TaqMan real-time PCR. Both methods showed a high degree of linearity (R(2) = ~1), although TaqMan real-time PCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might represent a promising platform for detecting PCV-2 viral loads. |
| File Format | HTM / HTML |
| ISSN | 10406387 |
| Issue Number | 6 |
| Volume Number | 27 |
| e-ISSN | 19434936 |
| Journal | Journal of Veterinary Diagnostic Investigation |
| Language | English |
| Publisher | Sage Publications |
| Publisher Date | 2015-11-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Veterinary Medicine Circoviridae Infections Veterinary Circovirus Isolation & Purification Real-time Polymerase Chain Reaction Swine Diseases Diagnosis Animals China Virology Genetics Dna, Viral Analysis Limit Of Detection Methods Sensitivity And Specificity Swine Viral Load Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|