NDLI: Growth phase-dependent regulation of the global virulence regulator Rot in clinical isolates of Staphylococcus aureus.

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Growth phase-dependent regulation of the global virulence regulator Rot in clinical isolates of Staphylococcus aureus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jelsbak, Lotte Hemmingsen, Lene Donat, Stefanie Ohlsen, Knut Boye, Kit Westh, Henrik Ingmer, Hanne Frees, Dorte
Description	Country affiliation: Denmark Author Affiliation: Jelsbak L ( Faculty of Life Sciences, Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.)
Abstract	Current models for global virulence regulation in Staphylococcus aureus are mainly based on studies performed with only a limited number of laboratory strains derived from NCTC8325. In these strains the small regulatory RNA, RNAIII, has a central role in virulence gene regulation. Recently, RNAIII was suggested to control transcription of target genes partly by inhibiting translation of the transcriptional regulator Rot. The present study was undertaken to examine if the model for RNAIII/Rot-dependent virulence regulation is conserved among clinical strains. To this end, we used Rot antibodies to directly assess the amount of Rot protein in 4 well-characterized S. aureus laboratory strains (8325-4, COL, Newman, and UAMS-1) and in 9 strains of clinical origin (encompassing USA300 and Mu50). Additionally, the cellular amount of RNAIII and rot mRNA was determined in all strains. The experiments revealed considerable variation in the Rot and RNAIII levels between strains. However, in the majority of strains the cellular amount of Rot was inversely correlated to the RNAIII level. As we demonstrate that Rot is a stable protein and that the level of rot transcript appeared similar in all strains, our data support that the model for RNAIII-mediated inhibition of rot mRNA translation is conserved among clinical strains. Assessment of Rot-dependent regulation of target genes revealed that Rot is a positive regulator of spa (protein A) transcription in all strains examined. In contrast, Rot repression of sspA (serine protease) and hlb (beta-hemolysin) transcription was not conserved between strains. From this study, we conclude that while the paradigm for understanding RNAIII-dependent regulation of Rot is well-conserved, regulation of single genes is subject to considerable strain variation. We propose that variation in global regulatory networks contribute considerably to the phenotypic variation observed between S. aureus isolates.
File Format	HTM / HTML
ISSN	14384221
Issue Number	4
Volume Number	300
e-ISSN	16180607
Journal	International Journal of Medical Microbiology
Language	English
Publisher	Elsevier
Publisher Date	2010-04-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Bacterial Proteins Biosynthesis Gene Expression Regulation, Bacterial Repressor Proteins Staphylococcus Aureus Physiology Bacterial Toxins Blotting, Western Gene Expression Profiling Hemolysin Proteins Humans Rna, Bacterial Serine Endopeptidases Sphingomyelin Phosphodiesterase Staphylococcal Infections Microbiology Staphylococcal Protein A Growth & Development Isolation & Purification Virulence Factors Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology Microbiology (medical)

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