NDLI: Identification and characterization of episomal forms of integrative genomic islands in the genus Francisella.

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Identification and characterization of episomal forms of integrative genomic islands in the genus Francisella.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rydzewski, Kerstin Tlapák, Hana Niehaus, Indra P. Dabrowski, Piotr W. Grunow, Roland Heuner, Klaus
Description	Country affiliation: Germany Author Affiliation: Rydzewski K ( Cellular Interactions of Bacterial Pathogens, Centre for Biological Threats and Special Pathogens, ZBS 2, Robert Koch Institute, Berlin, Germany.); Tlapák H ( Cellular Interactions of Bacterial Pathogens, Centre for Biological Threats and Special Pathogens, ZBS 2, Robert Koch Institute, Berlin, Germany.); Niehaus IP ( Cellular Interactions of Bacterial Pathogens, Centre for Biological Threats and Special Pathogens, ZBS 2, Robert Koch Institute, Berlin, Germany.); Dabrowski PW ( Centre for Biological Threats and Special Pathogens, Highly Pathogenic Viruses, ZBS 1, Robert Koch Institute, Berlin, Germany.); Grunow R ( Centre for Biological Threats and Special Pathogens, Highly Pathogenic Microorganisms, ZBS 2, Robert Koch Institute, Berlin, Germany.); Heuner K ( Cellular Interactions of Bacterial Pathogens, Centre for Biological Threats and Special Pathogens, ZBS 2, Robert Koch Institute, Berlin, Germany. Electronic address: HeunerK@rki.de.)
Abstract	Recently, we identified a putative prophage on a genomic island (GI) within the genome sequence of Francisella hispaniensis isolate AS0-814 (Francisella tularensis subsp. novicida-like 3523) by the analysis of the CRISPR-Cas systems of Francisella. Various spacer DNAs within the CRISPR region of different F. tularensis subsp. novicida strains were found to be homologous to the putative prophage (Schunder et al., 2013, Int. J. Med. Microbiol. 303:51-60). Now we identified the GI (FhaGI-1) as a mobile element which is able to form a circular episomal structure. The circular episomal form of FhaGI-1 is generated by F. hispaniensis, and the excision of the island is an integrase-dependent and site-specific process. Furthermore, we could demonstrate that the excision of the island is also possible in other bacterial species (Escherichia coli). In addition, we could show that a genetically generated small variant of the island is also functional and, after its electroporation into strain F. tularensis subsp. holarctica LVS, the GI was stable and site-specifically integrated into the genome of the transformants. The integrase is sufficient for the integration and excision of the small variant into and from the DNA backbone, respectively. Thus, the element may be suitable to be used as a genetic tool in F. tularensis research. Furthermore, we identified the tRNA(Val) gene of Francisella as an integration site for GIs. Genomic island FphGI-1 was identified in Francisella philomiragia ATCC 25016. We were not able to detect the episomal form of this GI, probably due to a mutated attR site. However, we could demonstrate that integrative GIs are present in Francisella and that they may allow horizontal gene transfer between different Francisella species.
File Format	HTM / HTML
ISSN	14384221
Issue Number	8
Volume Number	305
e-ISSN	16180607
Journal	International Journal of Medical Microbiology
Language	English
Publisher	Elsevier
Publisher Date	2015-12-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Francisella Genetics Genomic Islands Plasmids Escherichia Coli Integrases Metabolism Interspersed Repetitive Sequences Prophages Rna, Transfer, Val Recombination, Genetic Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology Microbiology (medical)

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