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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Du, Lili Xu, Xiaoming Duan, Xin Lu, Guangxiu Lin, Ge |
| Description | Author Affiliation: Du L ( Institute of Reproductive and Stem Cell Engineering, Central South University, 110 Xiang-Ya Road, Changsha, 410078, Hunan, People's Republic of China.) |
| Abstract | Human parthenogenetic embryonic stem (pES) cells can be clinically used in the future to avoid immunological rejection. However, the developmental potential of human pES cells remains to be elucidated. In this study, we generated a human pES-enhanced green fluorescent protein (EGFP) cell line (chHES-32-EGFP), which shows pluripotency thus far and maintains stable and robust EGFP expression in the undifferentiated and differentiated states in vivo and in vitro. Using this pES-EGFP cell line, we found that when human pES-EGFP cells were injected into mice blastocysts, EGFP-positive cells progressively decreased with the development of blastocysts in vitro. Only 4 out of 23 embryos (17.4%) contained EGFP-positive cells and all of these embryos exhibited abnormal morphology or delayed development when the chimera blastocysts were implanted into the pseudopregnant recipient mouse uterus. These results raise serious questions regarding the feasibility of the generation of interspecific chimeras between mouse blastocysts and human pES cells. |
| File Format | HTM / HTML |
| ISSN | 10712690 |
| Issue Number | 3 |
| Volume Number | 48 |
| e-ISSN | 1543706X |
| Journal | In Vitro Cellular & Developmental Biology - Animal |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2012-03-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Cell Biology Discipline Zoology Blastocyst Cytology Embryonic Stem Cells Green Fluorescent Proteins Analysis Pluripotent Stem Cells Animals Chimera Growth & Development Embryo Transfer Methods Genetics Humans Mice Parthenogenesis Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Medicine |
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