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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Farghaly, T. Khalifa, E. Mostafa, S. Hussein, M. Bedaiwy, M. Ahmady, A. |
| Description | Country affiliation: Egypt Author Affiliation: Farghaly T ( Obstetrics and Gynecology Department, Assiut University, Assiut University Street, Assiut, 71515, Egypt.) |
| Abstract | The aim of this study was to investigate the effect of delaying maturation by extended culture of immature bovine oocytes in prematuration medium (PMC) containing single maturation inhibitor on their meiotic resumption and embryonic development. Bovine immature oocytes were cultured in M199 containing 10 µM of either inhibitor (roscovitine, cilostamide, or forskolin) for either 72 or 120 h followed by up to 48 h in maturation media supplemented with 7.5 IU follicle-stimulating hormone (FSH)/luteinizing hormone (LH). Two control groups were used. In untreated control, immature oocytes were cultured in the same medium as the experimental group without any inhibitors. In the FSH/LH control group, oocytes were cultured directly in the maturation medium supplemented with FSH/LH up to 48 h. In vitro matured oocytes were then inseminated with frozen-thawed bull sperm. Fertilization, defined as two-cell division 48 h post-insemination, and blastocyst formation were recorded. Total maturation rate for the 72-h group was 73%, 70%, 66%, and 69% for roscovitine, forskolin, cilostamide, and FSH/LH control, respectively, with no significant difference indicating that inhibitors have no negative effect on the oocyte maturation rate. The total fertilization rate for the 72-h group revealed that cilostamide (47%) and roscovitine (35%) were significantly higher than FSH/LH control (20%). The total blastocyst formation rates per inseminated oocytes revealed that among treatment groups, roscovitine (20%) had significantly higher rate than forskolin (9%). Overall, 72-h exposure period had better outcomes than 120 h in all the treated groups. In conclusion, prematuration culture of the bovine oocytes in the presence of maturation inhibitor for 72-h period at 10 µM concentration is sufficient in improving the bovine oocyte developmental competence. |
| File Format | HTM / HTML |
| ISSN | 10712690 |
| Issue Number | 7 |
| Volume Number | 51 |
| e-ISSN | 1543706X |
| Journal | In Vitro Cellular & Developmental Biology - Animal |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2015-08-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Cell Biology Discipline Zoology In Vitro Oocyte Maturation Techniques Methods Meiosis Drug Effects Oocytes Cytology Animals Blastocyst Physiology Cattle Cells, Cultured Colforsin Pharmacology Culture Media Chemistry Female Fertilization In Vitro Follicle Stimulating Hormone Luteinizing Hormone Male Purines Quinolones Time Factors Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Medicine |
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