NDLI: Detection and confirmation of 60 anabolic and androgenic steroids in equine plasma by liquid chromatography-tandem mass spectrometry with instant library searching.

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Detection and confirmation of 60 anabolic and androgenic steroids in equine plasma by liquid chromatography-tandem mass spectrometry with instant library searching.

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Detection and confirmation of 60 anabolic and androgenic steroids in equine plasma by liquid chromatography-tandem mass spectrometry with instant library searching.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Ying Uboh, Cornelius E. Soma, Lawrence R. Li, Xiaoqing Guan, Fuyu You, Youwen Rudy, Jeffrey A. Chen, Jin-Wen
Description	Country affiliation: United States Author Affiliation: Liu Y ( University of Pennsylvania, New Bolton Center, Kennett Square, 19348, USA.)
Abstract	In 2008, Pennsylvania (PA) became the first State in the USA to ban and enforce the ban on the use of anabolic and androgenic steroids (AAS) in equine athletes by using plasma for analysis. To enforce the ban, a rapid and high-throughput method for analysis of 60 AAS in equine plasma was developed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analytes were recovered from plasma by liquid-liquid extraction (LLE) using methyl tert-butyl ether, separated on a reversed-phase C18 column and analyzed by electrospray ionization mass spectrometry. Multiple-reaction monitoring (MRM) scan was employed for screening. When the MRM signal of an analyte exceeded 1000 counts per second (cps), information-dependent acquisition (IDA) triggered generation of an enhanced product ion (EPI) scan of the analyte. A library for the analytes was simultaneously established using the EPI spectrum. Unambiguous identification of any of the 60 AAS in a test sample was based on both the presence of MRM response within the correct retention time (t(R)) window and a qualitative match between EPI spectrum of the test sample and that of the reference drug standard stored in the library. Total analysis time was 7 min. The limit of detection (LOD) and limit of confirmation (LOC) for most of the analytes were 0.01-2 ng/mL and 0.1-10 ng/mL, respectively. Recovery of the analytes from plasma by LLE was 74-138%. The method was successfully verified and is routinely used in the screening of post-race equine plasma samples for the presence of these 60 AAS. The method is rapid, sensitive, reproducible, and reliable.
File Format	HTM / HTML
ISSN	19427603
Issue Number	1
Volume Number	3
e-ISSN	19427611
Journal	Drug Testing and Analysis
Language	English
Publisher	Wiley
Publisher Date	2011-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Pharmacology Anabolic Agents Blood Androgens Chromatography, High Pressure Liquid Veterinary Data Mining Doping In Sports High-throughput Screening Assays Horses Performance-enhancing Substances Substance Abuse Detection Tandem Mass Spectrometry Chemistry Pharmacokinetics Animals Biological Markers Biotransformation Databases, Factual Molecular Structure Reproducibility Of Results Species Specificity Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Pharmaceutical Science

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