NDLI: Validated enzyme-linked immunosorbent assay for determination of rosuvastatin in plasma at picogram level.

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Validated enzyme-linked immunosorbent assay for determination of rosuvastatin in plasma at picogram level.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Darwish, Ibrahim A. Al-Obaid, Abdul-Rahman M. Al-Malaq, Hamoud A.
Description	Country affiliation: Saudi Arabia Author Affiliation: Darwish IA ( Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia. idarwish@ksu.edu.sa)
Abstract	In this study, a highly sensitive enzyme-linked immunosorbent assay (ELISA) has been developed and validated for the determination of rosuvastatin (ROS) in plasma samples at picogram level. The assay employed a polyclonal antibody that specifically recognizes ROS with high affinity, and ROS conjugate of bovine serum albumin (ROS-BSA) immobilized onto microplate wells as a solid phase. The assay involved a competitive binding reaction between ROS, in plasma sample, and the immobilized ROS-BSA for the binding sites on a limited amount of the anti-ROS antibody. The bound anti-ROS antibody was quantified with horseradish peroxidase-labelled second anti-rabbit IgG antibody (HRP-IgG) and 3,3`,5,5`-tetramethylbenzidine (TMB) as a substrate for the peroxidase enzyme. The concentration of ROS in the sample was quantified by its ability to inhibit the binding of the anti-ROS antibody to the immobilized ROS-BSA and subsequently the colour intensity in the assay wells. The assay limit of detection was 25 pg ml(-1) and the effective working range at relative standard deviations (RSD) of ≤ 5% was 40-2000 pg ml(-1). Analytical recovery of ROS from spiked plasma was 96.2 - 104.8 ± 2.12 - 5.42%. The precision of the assay was satisfactory; RSD was 2.47 - 4.46 and 3.24 - 5.27% for the intra- and inter-assay precision, respectively. The analytical procedure is convenient, and one can analyze ~ 200 samples per working day, facilitating the processing of large-number batch of samples. The proposed ELISA has a great value in routine analysis of ROS for its pharmacokinetic studies.
File Format	HTM / HTML
ISSN	19427603
Issue Number	5
Volume Number	5
e-ISSN	19427611
Journal	Drug Testing and Analysis
Language	English
Publisher	Wiley
Publisher Date	2013-05-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Pharmacology Fluorobenzenes Blood Hydroxymethylglutaryl-coa Reductase Inhibitors Pyrimidines Sulfonamides Animals Antibodies Analysis Binding, Competitive Cattle Enzyme-linked Immunosorbent Assay Methods Chemistry Horseradish Peroxidase Immobilized Proteins Immunoglobulin G Limit Of Detection Rabbits Rosuvastatin Calcium Serum Albumin, Bovine Journal Article Research Support, Non-u.s. Gov't Validation Studies
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Pharmaceutical Science

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