| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Douny, Caroline Bayram, Pinar Brose, François Degand, Guy Scippo, Marie-Louise |
| Description |
Country affiliation: Belgium Author Affiliation: Douny C ( Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, Quartier Vallèe 2, Avenue de Cureghem 10, Sart Tilman B43bis - 4000, Liège, Belgium.); Bayram P ( Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, Quartier Vallèe 2, Avenue de Cureghem 10, Sart Tilman B43bis - 4000, Liège, Belgium.); Brose F ( Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, Quartier Vallèe 2, Avenue de Cureghem 10, Sart Tilman B43bis - 4000, Liège, Belgium.); Degand G ( Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, Quartier Vallèe 2, Avenue de Cureghem 10, Sart Tilman B43bis - 4000, Liège, Belgium.); Scippo ML ( Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, Quartier Vallèe 2, Avenue de Cureghem 10, Sart Tilman B43bis - 4000, Liège, Belgium.) |
| Abstract |
Knowing that polyunsaturated fatty acids can lead to the formation of potentially toxic aldehydes as secondary oxidation products, an analytical method using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) detection was developed to measure the concentration of eight aldehydes in animal feed: malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), crotonaldehyde (CRT), benzaldehyde (BNZ), hexanal (HXL), 2,4-nonadienal, and 2,4-decadienal. The developed method was validated according to the criteria and procedure described in international standards. The evaluated parameters were specificity/selectivity, recovery, precision, accuracy, uncertainty, limits of detection and quantification, using the concept of accuracy profiles. These parameters were determined during experiments conducted over three different days with ground Kellogg's® Corn Flakes® cereals as model matrix for animal feed and spiked at different levels of concentration. Malondialdehyde, 4-HHE, 4-HNE, crotonaldehyde, benzaldehyde, and hexanal can be analyzed in the same run in animal feed with a very good accuracy, with recovery rates ranging from 86 to 109% for a working range going from 0.16 to 12.50 mg/kg. The analysis of 2,4-nonadienal and 2,4-decadienal can also be performed but in a limited range of concentration and with a limited degree of accuracy. Their recovery rates ranged between 54 and 114% and coefficient of variation for the intermediate precision between 11 and 25% for these two compounds. Copyright © 2016 John Wiley & Sons, Ltd. |
| File Format |
HTM / HTML |
| ISSN |
19427603 |
| Issue Number |
5-6 |
| Journal |
Drug Testing and Analysis |
| Volume Number |
8 |
| e-ISSN |
19427611 |
| Language |
English |
| Publisher |
Wiley |
| Publisher Date |
2016-05-01 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Pharmacology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Spectroscopy Environmental Chemistry Analytical Chemistry Pharmaceutical Science |
