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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ostroumova, Olga S. Chulkov, Evgeny G. Stepanenko, Olga V. Schagina, Ludmila V. |
| Description | Author Affiliation: Ostroumova OS ( Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia. Electronic address: osostroumova@mail.ru.); Chulkov EG ( Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.); Stepanenko OV ( Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.); Schagina LV ( Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.) |
| Abstract | Confocal fluorescence microscopy have been employed to investigate phase separation in giant unilamellar vesicles prepared from binary mixtures of unsaturated dioleoylphosphocholine with saturated phosphocholines or brain sphingomyelin in the absence and presence of the flavonoids, biochanin A, phloretin, and myricetin. It has been demonstrated that biochanin A and phloretin make uncolored domains more circular or eliminate visible phase separation in liposomes while myricetin remains the irregular shape of fluorescence probe-excluding domains. Influence of the flavonoids on the endotherms of liposome suspension composed of dioleoylphosphocholine and dimyristoylphosphocholine was investigated by the differential scanning calorimetry. Calorimetry data do not contradict to confocal imaging results. |
| File Format | HTM / HTML |
| ISSN | 00093084 |
| Volume Number | 178 |
| e-ISSN | 18732941 |
| Journal | Chemistry and Physics of Lipids |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-02-01 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Flavonoids Chemistry Lipid Bilayers Unilamellar Liposomes Calorimetry, Differential Scanning Fluorescent Dyes Microscopy, Confocal Phosphatidylcholines Sphingomyelins Transition Temperature Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Organic Chemistry Molecular Biology Biochemistry |
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