NDLI: Cell orientation and regulation of cell-cell communication in human mesenchymal stem cells on different patterns of electrospun fibers.

Please wait, while we are loading the content...

Year: 2017 Volume: 12

Year: 2016 Volume: 11

Year: 2015 Volume: 11

Year: 2015 Volume: 10

Year: 2014 Volume: 10

Year: 2014 Volume: 9

Year: 2013 Volume: 8

Year: 2013 Volume: 8 Issue: 6

Year: 2013 Volume: 8 Issue: 5

Magnesium-enhanced enzymatically mineralized platelet-rich fibrin for bone regeneration applications.

Cell orientation and regulation of cell-cell communication in human mesenchymal stem cells on different patterns of electrospun fibers.

Bio-inspired fabrication of fibroin cryogels from the muga silkworm Antheraea assamensis for liver tissue engineering.

Corrosion resistance and in vitro response of a novel Ti35Nb2Ta3Zr alloy with a low Young's modulus.

Spreading area and shape regulate apoptosis and differentiation of osteoblasts.

In vitro response of pre-osteoblastic cells to laser microgrooved PEEK.

Preparation and characterization of a hydrogel carrier to deliver gatifloxacin and its application as a therapeutic contact lens for bacterial keratitis therapy.

Enhanced osteogenic activity of a poly(butylene succinate)/calcium phosphate composite by simple alkaline hydrolysis.

Enhanced chemoresistance of squamous carcinoma cells grown in 3D cryogenic electrospun scaffolds.

Year: 2013 Volume: 8 Issue: 4

Year: 2013 Volume: 8 Issue: 3

Year: 2013 Volume: 8 Issue: 2

Year: 2013 Volume: 8 Issue: 1

Year: 2012 Volume: 7

Year: 2011 Volume: 6

Year: 2010 Volume: 5

Year: 2009 Volume: 4

Year: 2008 Volume: 3

Year: 2007 Volume: 2

Year: 2006 Volume: 1

Cell orientation and regulation of cell-cell communication in human mesenchymal stem cells on different patterns of electrospun fibers.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chang, Jui-Chih Fujita, Satoshi Tonami, Hiroyuki Kato, Koichi Iwata, Hiroo Hsu, Shan-hui
Description	Country affiliation: Taiwan Author Affiliation: Chang JC ( Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.)
Abstract	Cell behavior can be manipulated by the topography of the culture surface. In this study, we examined the intercellular communication and osteogenic differentiation of mesenchymal stem cells (MSCs) grown on electrospun fibers with different orientations and densities. Human bone marrow-derived MSCs (hMSCs) were seeded on poly(Îµ-caprolactone) (PCL) electrospun scaffolds composed of aligned (1D) or cross-aligned (2D) fibers (1.0-1.2 µm diameter) with high, medium, or low fiber densities. It was found that cells preferred to adhere onto electrospun PCL fibers rather than on the flat substrate. The immunofluorescence staining showed that the expression of vinculin, a focal adhesion protein, was limited to the periphery and the two extremities of aligned cells on the edge of the fibers. Electron microscopy showed that cells extended their lamellipodia across the adjacent fibers and proliferated along the direction of fibers. Cells grown on 1D fibrous scaffolds at all fiber densities had an obvious alignment. On 2D fibers, a higher degree of cell alignment was observed at the higher fiber density. On 1D scaffolds, the gap junction intercellular communication (GJIC) quantified by the lucifer yellow dye transfer assay was significantly promoted in the aligned cells in the direction parallel to the fibers but was abolished in the direction perpendicular to the fibers. The expression of osteogenic marker genes (RUNX2, ALP, and OCN) was significantly enhanced in seven days by culture on 1D but not 2D fibers. It was thus proposed that the promoted osteogenic differentiation of hMSCs may be associated with the fiber-guided and directional induction of GJIC.
File Format	HTM / HTML
ISSN	17486041
e-ISSN	1748605X
Journal	Biomedical Materials
Issue Number	5
Volume Number	8
Language	English
Publisher	IOP Publishing
Publisher Date	2013-10-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Biomedical Engineering Biocompatible Materials Chemistry Cell Communication Mesenchymal Stromal Cells Cytology Tissue Scaffolds Actins Metabolism Bone Marrow Cells Cell Culture Techniques Cell Differentiation Cell Proliferation Equipment Design Gap Junctions Microscopy, Electron, Scanning Osteoblasts Osteogenesis Polyesters Tissue Engineering Vinculin Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Chemistry Business and International Management Mechanics of Materials Biomaterials Bioengineering Biomedical Engineering

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in