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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ding, Shou-Nian Shan, Dan Xue, Huai-Guo Cosnier, Serge |
| Description | Country affiliation: China Author Affiliation: Ding SN ( School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, China. snding@seu.edu.cn) |
| Abstract | Nano-structured bismuth oxide (nano-BiOx) is a suitable material for enzyme immobilization owing to its attractive properties, such as large specific surface area, suitable permeability of the resulting film, the high biocompatibility, and as well as photovoltaic effect from semiconductor nanoparticles. Thus, a new type of amperometric glucose biosensor based on nano-BiOx was constructed. The amperometric detection of glucose was assayed by potentiostating the GOD/nano-BiOx electrode at 0.5 V to oxidize the enzymatically generated hydrogen peroxide. The proposed biosensor provided a linear response to glucose over a concentration range of 1 x 10(-6) M to 1.5 x 10(-3) M with a sensitivity of 51.0+/-0.4 mA/(M cm(2)) and a detection limit of 4 x 10(-7) M based on S/N=3. The apparent Michaelis-Menten constant was calculated to be 2.9 x 10(-3) M. In addition, characterization of nano-BiOx and modified electrode was performed by FT-IR spectroscopy, Raman spectroscopy, scanning electron microscope (SEM) and rotating-disk electrode (RDE) voltammetry. |
| File Format | HTM / HTML |
| ISSN | 15675394 |
| Issue Number | 2 |
| Volume Number | 79 |
| e-ISSN | 1878562X |
| Journal | Bioelectrochemistry |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-10-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Biosensing Techniques Methods Bismuth Chemistry Glucose Analysis Electrochemistry Electrodes Enzymes, Immobilized Metabolism Glucose Oxidase Hydrogen Peroxide Limit Of Detection Microscopy, Electron, Scanning Spectroscopy, Fourier Transform Infrared Spectrum Analysis, Raman Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Physical and Theoretical Chemistry Biophysics Electrochemistry |
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