NDLI: Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells.

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Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells.

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Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huang, Yan Jia, Xiaoling Bai, Ke Gong, Xianghui Fan, Yubo
Description	Country affiliation: China Author Affiliation: Huang Y ( Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Yue Yuan Road no. 37, Beijing, China.)
Abstract	BACKGROUND AND AIMS: Bone marrow mesenchymal stem cells (BMSCs) are a potential source of material for the construction of tissue-engineered cardiac grafts because of their potential to transdifferentiate into cardiomyocytes after chemical treatment or co-culture with cardiomyocytes. Recent evidence has shown that mechanical loads could regulate the BMSC differentiation into osteoblasts and endothelial cells through various signaling pathways. We investigated whether fluid shear stress (FSS), which is a mechanical load generated by fluid flow, can regulate rat BMSC (rBMSC) differentiation into cardiomyocytes. METHODS: rBMSCs were isolated from marrow of rat femur and tibia using density gradient centrifugation combined with adhesion method and identified with surface marker, proliferation character and differentiation potential in vitro. Cultured rBMSCs with or without 5-azacytidine (5-aza) treatment were exposed to laminar shear stress with a parallel plate-type device and analyzed by RT-PCR, immunocytochemistry, FACS and Western-blotting for the cardiomyogenic differentiation. RESULTS: Appropriate FSS treatment alone induced cardiomyogenic differentiation of rBMSCs, as confirmed by the expression of cardiomyocyte-related markers at both mRNA and protein levels. Furthermore, when rBMSC cultures were exposed to both FSS and 5-aza, expression levels of cardiomyocyte-related markers significantly increased to a degree suggestive of a synergistic interaction. CONCLUSIONS: The results demonstrate that FSS is an important factor affecting cardiomyogenic differentiation of rBMSCs. This provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated cells.
File Format	HTM / HTML
ISSN	01884409
Issue Number	7
Volume Number	41
e-ISSN	18735487
Journal	Archives of Medical Research
Language	English
Publisher	Elsevier
Publisher Date	2010-10-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Medicine Bone Marrow Cells Physiology Cell Differentiation Mesenchymal Stromal Cells Myocytes, Cardiac Stress, Mechanical Animals Cytology Cells, Cultured Cytoskeleton Metabolism Humans Male Mitogen-activated Protein Kinases Rats Rats, Sprague-dawley Shear Strength Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine

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