NDLI: Study of EWS/FLI-1 rearrangement in 18 cases of CK20+/CM2B4+ Merkel cell carcinoma using FISH and correlation to the differential diagnosis of Ewing sarcoma/peripheral neuroectodermal tumor.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fernandez-Flores, Angel Suarez-Peñaranda, Jose M. Alonso, Soledad
Description	Country affiliation: Spain Author Affiliation: Fernandez-Flores A ( *Service of Cellular Pathology, Clinica Ponferrada, Ponferrada Service of Anatomic Pathology, Complejo Hospitalario Universitario Santiago de Compostela Department of Medicine, University of Santiago de Compostela, Galicia §Department of Anatomic Pathology, Hospital Universitario de Guadalajara, Guadalajara, Spain.)
Abstract	Merkel cell carcinoma (MCC) and primary cutaneous Ewing sarcoma/primitive neuroectodermal tumors (PCES/PNET) pose a challenging morphologic differential diagnosis. Approximately 90% of Ewing sarcoma/primitive neuroectodermal tumors (PNETs) have a specific translocation, t(11;22) (q24;q12). The EWS-friend leukemia integration-1 (FLI-1) fusion results in FLI-1 overexpression. EWS/FLI-1 rearrangement has been suggested as a useful tool in the diagnosis of PCES/PNET. In contrast, Merkel cell polyomavirus was found to be an infective agent related to the pathogenesis of MCC. Merkel cell polyomavirus can be immunohistochemically detected with the antibody CM2B4. To the best of our knowledge, there is no case of any cytokeratin (CK)20-/CM2B4+ PNET. The goal of our study was to investigate whether EWS/FLI-1 rearrangement was present in cases of MCC. We have studied 18 cases of MCC. To make sure that the cases investigated by fluorescent in situ hybridization were genuine MCC, we considered only CK20+/CM2B4+ cases. Six cases met this criterion. EWS/FLI-1 rearrangement was not evidenced in any of the 18 cases (including the 6 'genuine' cases of MCC). Although our findings were somewhat expected, we think that they fill a gap in the literature: the confirmation that MCC is devoid of the EWS/FLI-1 rearrangement.
File Format	HTM / HTML
ISSN	10623345
Issue Number	5
Volume Number	21
e-ISSN	15334058
Journal	Applied Immunohistochemistry & Molecular Morphology
Language	English
Publisher	Lippincott Williams & Wilkins
Publisher Date	2013-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Histocytochemistry Carcinoma, Merkel Cell Diagnosis Genetics Chromosomes, Human, Pair 22 Neuroectodermal Tumors, Primitive, Peripheral Oncogene Proteins, Fusion Proto-oncogene Protein C-fli-1 Rna-binding Protein Ews Skin Neoplasms Aged Aged, 80 And Over Antigens, Viral Immunology Metabolism Chromosomes, Human, Pair 11 Diagnosis, Differential Female Humans Immunohistochemistry In Situ Hybridization, Fluorescence Keratin-20 Male Poliomyelitis Pathology Poliovirus Sarcoma, Ewing Journal Article
Content Type	Text
Resource Type	Article
Subject	Anatomy Medical Laboratory Technology

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