NDLI: BRIT1/MCPH1 expression in chronic myeloid leukemia and its regulation of the G2/M checkpoint.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Giallongo, C. Tibullo, D. La Cava, P. Branca, A. Parrinello, N. Spina, P. Stagno, F. Conticello, C. Chiarenza, A. Vigneri, P. Palumbo, G. A. Di Raimondo, F.
Description	Country affiliation: Italy Author Affiliation: Giallongo C ( Department of Clinical and Molecular Biomedicine, Section of Hematology, Ferrarotto Hospital, Via Citelli 6, Catania, Italy.)
Abstract	BRIT1 (BRCT-repeat inhibitor of hTERT expression), also known as microcephalin (MCPH1), is a crucial gene in the complex cellular machine that is devoted to DNA repair and acts as a regulator of both the intra-S and G2/M checkpoints. The most important role of BRIT1/MCPH1 in the regulation of cell cycle progression appears to be the G2/M checkpoint. The K562 and peripheral blood cells of chronic myeloid leukemia (CML) patients at diagnosis were found to downregulate BRIT1/MCPH1. However, we could not find any correlation between bcr/abl activity and the BRIT1/MCPH1 level. In order to study the genomic instability of CML cells, we evaluated the ability of these cells to arrest mitotic division after exposure to hydroxyurea, a known genotoxic agent. We showed that CML cells continue to proliferate without the activation of the G2/M cell cycle checkpoint arrest or of the apoptotic mechanism. This behavior may predispose the cells to accumulate genomic defects. In conclusion, we found that CML cells have a low BRIT1/MCPH1 level and show a defective G2/M arrest, confirming that these cells have a constitutive genomic instability.
File Format	HTM / HTML
ISSN	00015792
Issue Number	4
Volume Number	126
e-ISSN	14219662
Journal	Acta Haematologica
Language	English
Publisher	Karger
Publisher Date	2011-01-01
Publisher Place	Switzerland
Access Restriction	Subscribed
Subject Keyword	Discipline Hematology G2 Phase Cell Cycle Checkpoints Leukemia, Myelogenous, Chronic, Bcr-abl Positive Metabolism Neoplasm Proteins Nerve Tissue Proteins Actins Antagonists & Inhibitors Adult Aged Cell Proliferation Drug Effects Cells, Cultured Cytochalasin B Toxicity Cytokinesis Female Gene Expression Regulation, Neoplastic Genomic Instability Humans Hydroxyurea K562 Cells Blood Leukocytes Male Middle Aged Mutagens Genetics Rna, Messenger Journal Article
Content Type	Text
Resource Type	Article
Subject	Hematology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in