NDLI: Arf6 regulation of Gyrating-clathrin.

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Arf6 regulation of Gyrating-clathrin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Luo, Yi Zhan, Yi Keen, James H.
Description	Country affiliation: United States Author Affiliation: Luo Y ( Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA.)
Abstract	'Gyrating-' or 'G'-clathrin are coated endocytic structures located near peripheral sorting endosomes (SEs), which exhibit highly dynamic but localized movements when visualized by live-cell microscopy. They have been implicated in recycling of transferrin from the sorting endosome directly to the cell surface, but there is no information about their formation or regulation. We show here that G-clathrin comprise a minority of clathrin-coated structures in the cell periphery and are brefeldin A (BFA)-resistant. Arf6-GTP substantially increases G-clathrin levels, probably by lengthening coated bud lifetimes as suggested by photobleaching and photoactivation results, and an Arf6(Q67L)-GTP mutant bearing an internal GFP tag can be directly visualized in G-clathrin structures in live cells. Upon siRNA-mediated depletion of Arf6 or expression of Arf6(T27N), G-clathrin levels rise and are primarily Arf1-dependent, yet still BFA-resistant. However, BFA-sensitive increased G-clathrin levels are observed upon acute incubation with cytohesin inhibitor SecinH3, indicating a shift in GEF usage. Depletion of both Arf6 and Arf1 abolishes G-clathrin, and results in partial inhibition of fast transferrin recycling consistent with the latter's participation in this pathway. Collectively, these results demonstrate that the dynamics of G-clathrin primarily requires completion of the Arf6 guanine nucleotide cycle, but can be regulated by multiple Arf and GEF proteins, reflecting both overlapping mechanisms operative in their regulation and the complexity of processes involved in endosomal sorting.
File Format	HTM / HTML
ISSN	13989219
e-ISSN	16000854
DOI	10.1111/tra.12014
Journal	Traffic
Issue Number	1
Volume Number	14
Language	English
Publisher	Wiley
Publisher Date	2013-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Physiology Adp-ribosylation Factors Metabolism Clathrin-coated Vesicles Clathrin Adp-ribosylation Factor 1 Genetics Animals Brefeldin A Pharmacology Cos Cells Cercopithecus Aethiops Endosomes Guanine Nucleotide Exchange Factors Antagonists & Inhibitors Hela Cells Mutation, Missense Rna, Small Interfering Triazoles Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Structural Biology Molecular Biology Biochemistry

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Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake

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Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain.

Endosomal trafficking of the receptor tyrosine kinase MuSK proceeds via clathrin-dependent pathways, Arf6 and actin.

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