NDLI: Evaluation of Two PCR-Hybridization Methods for the Detection of Mycobacterium tuberculosis

Please wait, while we are loading the content...

Year: 2011 Volume: 31

Year: 2010 Volume: 30

Year: 2009 Volume: 29

Year: 2008 Volume: 28

Year: 2007 Volume: 27

Year: 2006 Volume: 26

Year: 2005 Volume: 25

Year: 2004 Volume: 24

Year: 2003 Volume: 23

Year: 2003 Volume: 23 Issue: 6

Year: 2003 Volume: 23 Issue: 5

Year: 2003 Volume: 23 Issue: 4

Year: 2003 Volume: 23 Issue: 3

Year: 2003 Volume: 23 Issue: 2

Year: 2003 Volume: 23 Issue: 1

Prenatal Diagnosis of Recurrent Trisomy 18 Syndrome

Distribution of Alleles and Clinical Manifestation in Patients with Progressive Ataxia Caused by Trinucleotide Repeat Expansion

Fatal Acute Hemolytic Transfusion Reaction Due to Anti-E+Fy(a)

Diversity of HLA-A26 Alleles and Haplotypes in Koreans

Diversity of HLA-B*40 Alleles and Haplotypes in Koreans

Serum Hepatitis B Virus DNA Quantitative Analysis Using Polymerase Chain Reaction in Patients with Chronic Hepatits B Virus Infection

Evaluation of Two PCR-Hybridization Methods for the Detection of Mycobacterium tuberculosis

Comparison of Three Radiolabeled Probes for PCR-Hybridization to Detect Mycobacterium tuberculosis

Characterization and Prevalence of Escherichia coli and Klebsiella pneumoniae Isolates Producing an Extended-Spectrum beta-Lactamase from Korean Hospitals

Semiquantitative Measurement of Hepatitis B Virus e Antigen by Elecsys 2010 Immunoanalyzer in Lamivudine Treated Patients

Reference Intervals of Reticulocyte Parameters using ADVIA 120

Clinical Relevance of Hyperleukocytosis in Patients with Acute Myelogenous Leukemia

Year: 2002 Volume: 22

Evaluation of Two PCR-Hybridization Methods for the Detection of Mycobacterium tuberculosis

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, J. H. Jang, S. J. Moon, D. S. Park, Y. J.
Description	Responsible library: WPRO
Abstract	BACKGROUND: We evaluated two homemade polymerase chain reaction-hybridization (PCRH) assays for the detection of M. tuberculosis. METHODS: Two PCRH assays, using a digoxigenin-luminescent probe (DL-PCRH) and using a radioactive probe (R-PCRH) were developed and compared. To determine the detection limit of each assay, 10-fold serial dilution samples of M. tuberculosis DNA were tested. To determine the specificity of each assay, 4 nontuberculous mycobacteria (NTM) and 13 bacteria other than mycobacteria were tested. Sputum samples from 50 patients with tuberculosis and 26 patients with nontuberculous diseases were tested by DL-PCRH, R-PCRH, culture, AFB stain and PCR assay methods, respectively. RESULTS: Both of the DL-PCRH and R-PCRH methods showed the same detection limit of 100 fg of M. tuberculosis DNA which was a log higher than that for the PCR. Sensitivity of both methods for the detection of M. tuberculosis in sputum specimens was 84%, which were slightly inferior to that of the culture (90%) and similar to that of the PCR (82%). However, both DL-PCRH and R-PCRH methods correctly identified M. tuberculosis for all six specimens that showed weakly-positive or negative PCR results. No false-positive results were obtained for patients with nontuberculous diseases (n=26), NTM (n=4) and other bacteria (n=13). CONCLUSIONS: Although the diagnostic efficiency of the two PCRH assays were similar to that of the PCR, both methods have greater objectivity in reading results than the PCR. This suggests that the PCRH is suitable for use in large number of clinical samples for the rapid detection of M. tuberculosis.
File Format	HTM / HTML
ISSN	22343806
e-ISSN	22343814
Journal	The Korean Journal of Laboratory Medicine
Issue Number	1
Volume Number	23
Language	Korean
Publisher	Korean Society for Laboratory Medicine
Publisher Date	2003-01-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Medical Laboratory Technology Pro-corticotropin Releasing Hormone Polymerase Chain Reaction Mycobacterium Tuberculosis Upper Case Are Lower Case En
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Clinical Biochemistry

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in