NDLI: Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test

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Analysis of Positive Results in Mediace Rapid Plasma Reagin and Treponema pallidum Latex Agglutination as the Automated Syphilis Test

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Clinical significance of quantitation of WT1 gene expression for minimal residual disease monitoring of acute myelogenous leukemia

Reconfirmation of preanalytical variables and establishment of reference intervals of platelet function analyzer-100 closure times in Korean adults

Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test

Evaluation of Viva-E drug testing system

Seven cases of decreased serum valproic acid concentration during concomitant use of carbapenem antibiotics

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Evaluation of BioSewoom HLA-A, -B, -C PCR/SSP kit

A RhD negative patient failed to produce detectable anti-D after transfusion of 35 units of RhD positive red blood cells

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Comparison of cardiovascular risk factors between normotension and prehypertension

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Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huh, H. J. Lee, K. K. Kim, E. S. Chae, S. L.
Description	Responsible library: WPRO
Abstract	BACKGROUND: We compared the results of automated and quantitative methods for the diagnosis of syphilis, Mediace Rapid Plasma Reagin (RPR) and Mediace Treponema pallidum Latex Agglutination (TPLA) (Sekisui Chemical Co., Ltd, Japan) with those of conventional methods. METHODS: Sera from 3,896 persons who had health checkups between December 2005 and November 2006 were included in the evaluation of positive rates and biological false positives (BFP) for Mediace RPR and TPLA. In addition, 134 patients' sera positive for automated Mediace RPR or TPLA were tested for VDRL and TPHA. Discrepancies between TPLA and TPHA results were confirmed by the RecomBlot Treponemal IgG/IgM (Mikrogen GmbH, Germany). Automated Mediace RPR and TPLA were performed using the Hitachi 7600 chemistry autoanalyzer (Hitachi, Japan). Samples with positive Mediace RPR and negative TPLA results were defined as BFP. RESULTS: Positive rate of automated Mediace RPR was 0.23% (9/3,896). BFP of the Mediace RPR was 0.18%. Positive rate of automated TPLA was 1.62% (37/2,284). Among the 134 patients' sera, 33 (24.6%) showed a discrepancy between conventional VDRL and automated Mediace RPR results: Among 31 Mediace RPR(+)/VDRL(-) sera, 13 were positive and 18 were negative for TPLA. The remaining 2 sera of discrepancy with Mediace RPR(-)/VDRL(+) were all positive for TPLA. There were seven sera that showed a discrepancy between automated TPLA and TPHA results: Two sera with Mediace RPR(+)/TPLA(-)/TPHA(+) showed negative recomBlot Treponemal IgG/IgM results, and among five sera with TPLA(+)/TPHA(-), three demonstrated IgG or IgM by recomBlot Treponemal IgG/IgM. CONCLUSIONS: The results of comparison data demonstrated that automated TPLA results had a high concordance with recomBlot Treponemal IgG/IgM results. Moreover, there are additional advantages of automated methods such as quantitative detection, low infection risk, and no influence by human handling.
File Format	HTM / HTML
ISSN	22343806
e-ISSN	22343814
Journal	The Korean Journal of Laboratory Medicine
Issue Number	5
Volume Number	27
Language	Korean
Publisher	Korean Society for Laboratory Medicine
Publisher Date	2007-01-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Medical Laboratory Technology
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Clinical Biochemistry

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