NDLI: Epstein-Barr virus and breast cancer: lack of evidence for an association in Iranian women.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kadivar, Maryam Monabati, Ahmad Joulaee, Azadeh Hosseini, Niloufar
Spatial Coverage	Iran
Description	Country affiliation: Iran Author Affiliation: Kadivar M ( Department of Pathology, Tehran University of Medical Sciences, Hazrat Rasool-e-Akram Hospital, Tehran, Iran. dmkadivar@gmail.com)
Abstract	Controversies regarding the role of Epstein-Barr virus (EBV) in breast cancer and lack of published literature in this regard in Iran, prompted us to assess EBV presence in 100 breast carcinoma and 42 control biopsies obtained from Iranian women. Breast carcinoma cases were comprised of 81 invasive ductal carcinoma NOS, 9 invasive lobular carcinoma, 1 apocrine carcinoma, 2 cribriform carcinoma, 2 papillary carcinoma and 5 mucinous carcinoma. Control biopsies consisted of 13 fibroadenoma, 9 benign epithelial proliferation (adenosis and sclerosing adenosis), 9 usual ductal hyperplasia, 4 atypical ductal hyperplasia, 4 non-proliferative fibrocystic changes and 3 normal breast tissue. To identify EBV-infected cells we applied immunohistochemical analysis, using monoclonal antibody against Epstein-Barr virus-encoded nuclear antigen 2 (EBNA-2) and latent membrane protein 1 (LMP-1). Further, polymerase chain reaction (PCR) was used to amplify EBV DNA, with primers that cover the EBV encoded RNA (EBER) and BamHIW regions. EBNA-2 and LMP-1 immunohistochemistry were negative in all breast cancer and control specimens. Using PCR, none of the 100 breast cancer samples or the 42 control specimens showed detectable EBV DNA. These results indicate that EBV may not play a significant role in the etiology of breast cancer in Iranian women.
File Format	HTM / HTML
ISSN	12194956
Issue Number	3
Volume Number	17
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2011-09-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Breast Neoplasms Diagnosis Carcinoma, Ductal, Breast Carcinoma, Lobular Epstein-barr Virus Infections Epstein-barr Virus Nuclear Antigens Genetics Viral Matrix Proteins Viral Proteins Adolescent Adult Breast Metabolism Pathology Virology Dna, Viral Female Herpesvirus 4, Human Humans Immunoenzyme Techniques Iran Middle Aged Polymerase Chain Reaction Young Adult Comparative Study Journal Article Randomized Controlled Trial
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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