NDLI: Intravenous administration of a single-dose free-circulating DNA of colitic origin improves severe murine DSS-colitis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sipos, Ferenc Muzes, Györgyi Furi, István Spisák, Sándor Wichmann, Barnabás Germann, Tiana M. Constantinovits, Miklós Krenács, Tibor Tulassay, Zsolt Molnár, Béla
Description	Author Affiliation: Sipos F ( Cell Analysis Laboratory, 2nd Department of Internal Medicine, Semmelweis University, Szentkirályi Street 46, 1088, Budapest, Hungary, dr.siposf@gmail.com.)
Abstract	In inflammatory bowel diseases the presence of free-circulating DNA (fcDNA) sequences in the sera is an established phenomenon, albeit its real biological function still remains unclear. In our study the immunobiologic effects of a single-dose, intravenously administered fcDNA of normal and colitic origin were assayed in DSS-colitic and control mice. In parallel with disease and histological activity evaluations changes of the TLR9 and inflammatory cytokine signaling gene expression profiles were assayed in isolated cells of the lamina propria. Intravenously administered colitis-derived fcDNA displayed a more prominent beneficial action regarding the clinical and histological severity of DSS-colitis than that of fcDNA of normal origin. Systemic administration of colitis-derived fcDNA significantly altered the expression of certain TLR9-related and proinflammatory cytokine genes in a clinically favorable manner. Presumably due to induction of severe colitis, the subsequent marked inflammatory environment may result changes in fcDNA with a potential to promote the downregulation of inflammation and improvement of tissue regeneration. Elucidating mechanisms of innate immune alterations by nucleic acids may provide further insight into the etiology of inflammatory bowel diseases, and develop the basis of novel nucleic acid-based immunotherapies.
File Format	HTM / HTML
ISSN	12194956
Issue Number	4
Volume Number	20
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2014-10-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Colitis Chemically Induced Prevention & Control Dna Pharmacology Dextran Sulfate Toxicity Disease Models, Animal Inflammation Administration, Intravenous Animals Pathology Administration & Dosage Blood Gene Expression Regulation Immunology Male Mice Mice, Inbred C57bl Rna, Messenger Genetics Real-time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Toll-like Receptor 9 Metabolism Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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