NDLI: Cel8H, a novel endoglucanase from the halophilic bacterium Halomonas sp. S66-4: molecular cloning, heterogonous expression, and biochemical characterization.

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Cel8H, a novel endoglucanase from the halophilic bacterium Halomonas sp. S66-4: molecular cloning, heterogonous expression, and biochemical characterization.

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Cel8H, a novel endoglucanase from the halophilic bacterium Halomonas sp. S66-4: molecular cloning, heterogonous expression, and biochemical characterization.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huang, Xiaoluo Shao, Zongze Hong, Yuzhi Lin, Ling Li, Chanjuan Huang, Fei Wang, Hui Liu, Ziduo
Description	Author Affiliation: Huang X ( State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.)
Abstract	A recombinant Escherichia coli clone expressing an endoglucanase was identified from a genomic library of the halophilic bacterium Halomonas sp. S66-4, and the enzyme was designated Cel8H. The cel8H gene consisted of 1,053 bp and encoded 350 amino acids sharing the highest identity of 48% to other known endoglucanases. The protein was expressed in E. coli BL21 (DE3) and purified to homogeneity. The purified recombinant enzyme had an optimal activity of 4.9 U/mg at pH 5 and 45 degrees C toward the substrate carboxymethylcellulose. It exhibited extraordinary properties which differed from endoglucanases reported previously at the point of high salt tolerance above 5 M, simultaneously with high pH stability at pH 4-12 and high temperature stability at 40-60 degrees C. Various substrate tests indicated that the enzyme hydrolyzes beta-1,4-glucosidic bonds specifically.
File Format	HTM / HTML
ISSN	12258873
Issue Number	3
Volume Number	48
e-ISSN	19763794
Journal	Journal of Microbiology
Language	English
Publisher	Springer
Publisher Date	2010-06-01
Publisher Place	Korea (South)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Bacterial Proteins Genetics Cellulase Genes, Bacterial Halomonas Enzymology Amino Acid Sequence Metabolism Base Sequence Carboxymethylcellulose Sodium Cloning, Molecular Dna Primers Dna, Bacterial Enzyme Stability Escherichia Coli Genomic Library Hydrogen-ion Concentration Kinetics Molecular Sequence Data Recombinant Proteins Sequence Homology, Amino Acid Species Specificity Substrate Specificity Temperature Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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