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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Han, Jiwon Jung, Jaejoon Hyun, Seunghun Park, Hyun Park, Woojun |
| Spatial Coverage | Antarctic Regions |
| Description | Author Affiliation: Han J ( Department of Environmental Science and Ecological Engineering, Korea University, Seoul 136-713, Republic of Korea.) |
| Abstract | Pollution of Antarctic soils may be attributable to increased nutritional input and diesel contamination via anthropogenic activities. To investigate the effect of these environmental changes on the Antarctic terrestrial ecosystem, soil enzyme activities and microbial communities in 3 types of Antarctic soils were evaluated. The activities of alkaline phosphomonoesterase and dehydrogenase were dramatically increased, whereas the activities of ß-glucosidase, urease, arylsulfatase, and fluorescein diacetate hydrolysis were negligible. Alkaline phosphomonoesterase and dehydrogenase activities in the 3 types of soils increased 3- to 10-fold in response to nutritional input, but did not increase in the presence of diesel contamination. Consistent with the enzymatic activity data, increased copy numbers of the phoA gene, encoding an alkaline phosphomonoesterase, and the 16S rRNA gene were verified using quantitative real-time polymerase chain reaction. Interestingly, dehydrogenase activity and 16S rRNA gene copy number increased slightly after 30 days, even under diesel contamination, probably because of adaptation of the bacterial population. Intact Antarctic soils showed a predominance of Actinobacteria phylum (mostly Pseudonorcarida species) and other phyla such as Proteobacteria, Chloroflexi, Planctomycetes, Firmicutes, and Verrucomicrobia were present in successively lower proportions. Nutrient addition might act as a selective pressure on the bacterial community, resulting in the prevalence of Actinobacteria phylum (mostly Arthrobacter species). Soils contaminated by diesel showed a predominance of Proteobacteria phylum (mostly Phyllobacterium species), and other phyla such as Actinobacteria, Bacteroidetes, Planctomycetes, and Gemmatimonadetes were present in successively lower proportions. Our data reveal that nutritional input has a dramatic impact on bacterial communities in Antarctic soils and that diesel contamination is likely toxic to enzymes in this population. |
| File Format | HTM / HTML |
| ISSN | 12258873 |
| Issue Number | 6 |
| Volume Number | 50 |
| e-ISSN | 19763794 |
| Journal | Journal of Microbiology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2012-12-01 |
| Publisher Place | Korea (South) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Enzymes Chemistry Gasoline Soil Microbiology Soil Alkaline Phosphatase Genetics Antarctic Regions Bacteria Classification Biodiversity Dna Ecosystem Environment Adverse Effects Gene Dosage Metagenome Oxidoreductases Phylogeny Rna, Ribosomal, 16s Soil Pollutants Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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