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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Evenou, Fanny Couderc, Sandrine Kim, Beomjoon Fujii, Teruo Sakai, Yasuyuki |
| Description | Country affiliation: Japan Author Affiliation: Evenou F ( Laboratory for Integrated Micro-Mechatronic Systems (LIMMS/CNRS-IIS), Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. fanny.evenou@univ-paris-diderot.fr) |
| Abstract | Here we report the use of natural, chemically-unmodified, microfibrillated cellulose (MFC) as a matrix for hepatocyte culture. We developed an original cell-culture design composed of a thin 3D-microstructured fibrous substrate consisting of a MFC sheet coating a highly $O_{2}-permeable$ polydimethylsiloxane (PDMS) membrane. The MFC-coated PDMS membranes were obtained according to a simple process where cellulose fibres were deposited from an aqueous suspension on the PDMS surfaces and the films were dried under mild conditions. To enable oxygen diffusion through the membranes, they were assembled on bottomless frames $(`O_{2}+`$ condition). Rat hepatocytes primary-cultured on such MFC-PDMS membranes quickly organized themselves into large hemispherical 3D aggregates which were tightly anchored to the MFC sheets. In contrast, hepatocytes cultured on smooth PDMS membranes in the $O_{2}+$ system $(O_{2}+,$ PDMS) organized into unstable 2D monolayers which easily detached from the surfaces. Hepatocyte 3D cultures obtained on MFC-PDMS membranes exhibited higher liver-specific functions over a 2-week culture period, as assessed by both the higher albumin secretion and urea synthesis rate. The MFC-PDMS membranes appear suitable for obtaining stably-attached and functional hepatocyte 3D cultures and appear interesting for drug/chemical screenings in a microplate format, but also for microfluidic applications. |
| File Format | HTM / HTML |
| ISSN | 09205063 |
| Issue Number | 11 |
| Volume Number | 22 |
| e-ISSN | 15685624 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2011-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biomedical Engineering Cell Culture Techniques Methods Cellulose Chemistry Pharmacology Dimethylpolysiloxanes Hepatocytes Cytology Membranes, Artificial Oxygen Animals Cell Aggregation Drug Effects Diffusion Metabolism Liver Male Organ Specificity Permeability Rats Rats, Wistar Wood Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biomaterials Biophysics Bioengineering Biomedical Engineering |
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