NDLI: Catalase and superoxide dismutase double staining zymogram technique for Deinococcus and Kocuria species exposed to multiple stresses.

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Catalase and superoxide dismutase double staining zymogram technique for Deinococcus and Kocuria species exposed to multiple stresses.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shukla, Manish R. Yadav, Radhika Desai, Anjana
Description	Country affiliation: United States Author Affiliation: Shukla MR ( Department of Biological Sciences, Louisiana State University and A &M College, Baton Rouge, LA, USA.)
Abstract	Superoxide dismutase (SOD) and catalase expression is associated with oxidative stress. Existing techniques for the individual staining of SOD and catalase have been described in the past. The objective of this study was to achieve a simple and rapid technique for the double staining of bacterial SOD and catalase on the same polyacrylamide gel. SOD detection was carried out using nitro-blue tetrazolium (NBT) dye reduction followed by ferricyanide precipitation for negative staining of the catalase enzyme on the same gel. The staining procedure resulted in pale blue SOD bands while catalase appeared as yellow bands against a greenish blue background on the same gel. This technique was used to detect changes in the polymorphic forms of these enzymes in Deinococcus radiodurans R1 and Kocuria sp. C2 subjected to stresses like UV and gamma radiation and desiccation.
File Format	HTM / HTML
ISSN	0233111X
Issue Number	6
Volume Number	49
e-ISSN	15214028
Journal	Journal of Basic Microbiology
Language	English
Publisher	Wiley-VCH Verlag
Publisher Date	2009-12-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Catalase Metabolism Deinococcus Enzymology Micrococcaceae Staining And Labeling Methods Superoxide Dismutase Electrophoresis, Polyacrylamide Gel Ferricyanides Nitroblue Tetrazolium Oxidative Stress Protein Isoforms Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology

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Modifying Polyacrylamide Background Color for the Nitroblue Tetrazolium-Based Superoxide Dismutase Staining Assay

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Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.

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Inhibition of nitrergic neurotransmission in the bovine retractor penis muscle by an oxidant stress: effects of superoxide dismutase mimetics

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Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

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Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage

Catalase and superoxide dismutase double staining zymogram technique for Deinococcus and Kocuria species exposed to multiple stresses.

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Catalase and superoxide dismutase double staining zymogram technique for Deinococcus and Kocuria species exposed to multiple stresses.