NDLI: Defensins attenuate cytokine responses yet enhance antibody responses to Porphyromonas gingivalis adhesins in mice

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Defensins attenuate cytokine responses yet enhance antibody responses to Porphyromonas gingivalis adhesins in mice

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kohlgraf, Karl G. Ackermann, Abbey Lu, Xiaoying Burnell, Kindra Bélanger, Myriam Cavanaugh, Joseph E. Xie, Hua Progulske-Fox, Ann Brogden, Kim A.
Description	Country affiliation: United States Author Affiliation: Kohlgraf KG ( Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA. karl-kohlgraf@uiowa.edu)
Abstract	Aim Our aim is to assess the ability of human neutrophil peptide α-defensins (HNPs) and human β-defensins (HBDs) to attenuate proinflammatory cytokine responses and enhance antibody responses to recombinant hemagglutinin B (rHagB) or recombinant fimbrillin A (rFimA) from Porphyromonas gingivalis 381 in mice. Materials & methods In the first study, C57BL/6 mice were given 10 μg rHagB or rFimA without and with 1 μg HNP1, HNP2, HBD1, HBD2 or HBD3. At 24 h, mice were euthanized and cytokine concentrations were determined in nasal wash fluid (NWF), bronchoalveolar lavage fluids, saliva and serum. In the second study, C57BL/6 mice were given 10 μg rHagB or rFimA without and with 1 μg HNPs or HBDs similarly on days 0, 7 and 14. At 21 days, mice were euthanized and rHagB- and rFimA-specific antibody responses were determined in NWF, bronchoalveolar lavage fluids, saliva and serum. Results Mice given rHagB + HNP2, rHagB + HBD1 and rHagB + HBD3 produced significantly lower (p < 0.05) IL-6 responses than mice given rHagB alone. Mice given rHagB + HNP1, rHagB + HNP2, rHagB + HBD1 and rHagB + HBD3 produced significantly lower (p < 0.05) keratinocyte-derived chemokine responses than mice given rHagB alone. Mice given rFimA produced very low levels of IL-6 and only moderate levels of keratinocyte-derived chemokine in NWF that were not attenuated by prior incubation of rFimA with any defensin. Mice given rHagB + HNP1 produced a significantly higher (p < 0.05) serum IgG antibody response than mice given rHagB alone and mice given rFimA + HNP2 produced a higher, but not significant, antibody response. Conclusion The ability of HNPs and HBDs to attenuate proinflammatory cytokine responses in murine NWF and enhance IgG antibody responses in serum was dependent upon both the defensin and antigen of P. gingivalis.
File Format	HTM / HTML
ISSN	17460913
e-ISSN	17460921
DOI	10.2217/fmb.09.107
Journal	Future Microbiology
Issue Number	1
Volume Number	5
Language	English
Publisher	Future Medicine
Publisher Date	2010-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Adhesins, Bacterial Immunology Antibodies, Bacterial Bacteroidaceae Infections Cytokines Antagonists & Inhibitors Fimbriae Proteins Alpha-defensins Beta-defensins Animals Microbiology Pathology Bronchoalveolar Lavage Fluid Lectins Mice Mice, Inbred C57bl Nasal Cavity Porphyromonas Gingivalis Saliva Serum Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Microbiology Microbiology (medical)

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