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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Raghu, P. Madhusudana Reddy, T. Reddaiah, K. Jaidev, L. R. Narasimha, G. |
| Description | Country affiliation: India Author Affiliation: Raghu P ( Electrochemical Research Laboratory, Department of Chemistry, S.V.U. College of Sciences, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India.) |
| Abstract | A novel electrochemical biosensor for the determination of pyrogallol (PG) and hydroquinone (HQ) has been constructed based on the poly l-arginine (poly(l-Arg))/carbon paste electrode (CPE) immobilized with horseradish peroxidase (HRP) and silver nanoparticles (AgNPs) through the silica sol-gel (SiSG) entrapment. The electrochemical properties of the biosensor were characterized by employing the electrochemical techniques. The proposed biosensor showed a high sensitivity and fast response toward the determination of PG and HQ around 0.18V. Under the optimized conditions, the anodic peak current of PG and HQ was linear with the concentration range of 8µM to 30×10(-5)M and 1-150µM. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 6.2µM, 20µM for PG and 0.57µM, 1.92µM for HQ respectively. The electrochemical impedance spectroscopy (EIS) studies have confirmed that the occurrence of electron transfer at HRP-SiSG/AgNPs/poly(l-Arg)/CPE was faster. Moreover the stability, reproducibility and repeatability of the biosensor were also studied. The proposed biosensor was successfully applied for the determination of PG and HQ in real samples and the results were found to be satisfactory. |
| File Format | HTM / HTML |
| ISSN | 01410229 |
| Issue Number | 6-7 |
| Volume Number | 52 |
| e-ISSN | 18790909 |
| Journal | Enzyme and Microbial Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-05-10 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Biochemistry Discipline Biotechnology Biosensing Techniques Electrochemistry Methods Enzymes, Immobilized Metabolism Horseradish Peroxidase Hydroquinones Analysis Pyrogallol Arginine Electrodes Chemistry Nanoparticles Peptides Sensitivity And Specificity Silver Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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