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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Chia-Chen Reddy, P. Muralidhar Devi, C. Shobha Chang, Po-Chi Ho, Yen-Peng |
| Description | Country affiliation: Taiwan Author Affiliation: Chen CC ( Department of Chemistry, National Dong Hwa University, Hualien 97401, Taiwan.); Reddy PM ( Department of Chemistry, Nizam College, Osmania University, Hyderabad, India.); Devi CS ( Department of Chemistry, National Dong Hwa University, Hualien 97401, Taiwan.); Chang PC ( Department of Chemistry, National Dong Hwa University, Hualien 97401, Taiwan.); Ho YP ( Department of Chemistry, National Dong Hwa University, Hualien 97401, Taiwan. Electronic address: ypho@mail.ndhu.edu.tw.) |
| Abstract | The effect of microwave heating on lipase-catalyzed reaction remains controversial. It is not clear whether the reaction rate enhancements are purely due to thermal/heating effects or to non-thermal effects. Therefore, quantitative mass spectrometry was used to conduct accurate kinetic analysis of lipase-catalyzed hydrolysis of triolein by microwave and conventional heating. Commercial lipases from Candida rugosa (CRL), Porcine Pancreas (PPL), and Burkholderia cepacia (BCL) were used. Hydrolysis reactions were performed at various temperatures and pH levels, along with various amounts of buffer and enzymes. Hydrolysis product yields at each time point using an internal-standard method showed no significant difference between microwave and conventional heating conditions when the reaction was carried out at the same temperature. CRL showed optimum catalytic activity at 37 °C, while PPL and BCL had better activities at 50 °C. The phosphate buffer was found to give a better hydrolysis yield than the Tris-HCl buffer. Overall results prove that a non-thermal effect does not exist in microwave-assisted lipase hydrolysis of triolein. Therefore, conventional heating at high temperatures (e.g., 50 °C) can be also used to accelerate hydrolysis reactions. |
| File Format | HTM / HTML |
| ISSN | 01410229 |
| Volume Number | 82 |
| e-ISSN | 18790909 |
| Journal | Enzyme and Microbial Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Biochemistry Discipline Biotechnology Lipase Metabolism Microwaves Triolein Animals Bacterial Proteins Burkholderia Cepacia Enzymology Candida Fungal Proteins Hot Temperature Hydrolysis Mass Spectrometry Oleic Acid Analysis Pancreas Swine Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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